Molecular Cloning of a Novel Crustacean Member of the Aldoketoreductase Superfamily, Differentially Expressed in the Antennal Glands
| Autor: | M. Maı̈bèche-Coisne, S. Aragon, Chantal Dauphin-Villemant, M. Boscameric, R. Lafont |
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| Přispěvatelé: | Santiago, Aragon, Université Pierre et Marie Curie - Paris 6 (UPMC) |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
DNA
Complementary [SDV.BA] Life Sciences [q-bio]/Animal biology Molecular Sequence Data Aldo-Keto Reductases Biophysics Gene Expression Astacoidea In situ hybridization Biology Molecular cloning Biochemistry Molting cycle Open Reading Frames Aldehyde Reductase Complementary DNA Animals Amino Acid Sequence Northern blot Molecular Biology In Situ Hybridization chemistry.chemical_classification Base Sequence Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction [SDV.BA]Life Sciences [q-bio]/Animal biology Cell Biology Orconectes limosus Blotting Northern biology.organism_classification Molecular biology Amino acid Alcohol Oxidoreductases chemistry Function (biology) |
| Zdroj: | Biochemical and Biophysical Research Communications Biochemical and Biophysical Research Communications, 2001, 282, pp.314-320 |
| ISSN: | 0006-291X 1090-2104 |
| Popis: | Biochemical studies on ecdysteroid metabolism in arthropods suggest that aldoketoreductase enzymes (AKRs) may be involved in this pathway, but very few molecular data are available on these oxidoreductases in invertebrates. Looking for such enzymes in the crayfish Orconectes limosus, we have used a PCR strategy with primers deduced from a recent insect 3β-reductase sequence, and from mammalian 5β-reductase sequences. A full-length cDNA, corresponding to a putative AKR, was isolated from crayfish antennal gland. This cDNA contains an open-reading frame of 1008 bp, encoding a predicted protein of 336 amino acids. Northern blots indicated a restricted expression of the transcript in the antennal glands, quite constant during the molting cycle, and in situ hybridization demonstrated a strong expression of the transcript in the labyrinth. This is to date the first member of the AKRs superfamily characterized in a crustacean species, and the putative function of the corresponding enzyme is discussed. |
| Databáze: | OpenAIRE |
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