Gene expression profiling in an in vitro model of angiogenesis
| Autor: | Juliet E. Bryant, Mary E. Gerritsen, Fuad Mehraban, Xiaohua Xin, David A. Lewin, Franklin Peale, Aparna Draksharapu, Gladys Ingle, Gordon A. Vehar, Jeanne Kahn, Jill Schoenfeld, Chrisopher J. Grimaldi |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
DNA
Complementary Angiogenesis Gene Expression Neovascularization Physiologic Receptors Cytoplasmic and Nuclear DNA Fragmentation Biology Pathology and Forensic Medicine Veins Neovascularization Complementary DNA Gene expression medicine STC1 Humans Gene Cells Cultured In Situ Hybridization Genome GeneCalling Molecular biology DNA Fingerprinting Gene expression profiling Endothelium Vascular medicine.symptom Regular Articles Transcription Factors |
| Zdroj: | The American journal of pathology. 156(6) |
| ISSN: | 1525-2191 0002-9440 |
| Popis: | In the present study we have used a novel, comprehensive mRNA profiling technique (GeneCalling) for determining differential gene expression profiles of human endothelial cells undergoing differentiation into tubelike structures. One hundred fifteen cDNA fragments were identified and shown to represent 90 distinct genes. Although some of the genes identified have previously been implicated in angiogenesis, potential roles for many new genes, including OX-40, white protein homolog, KIAA0188, a homolog of angiopoietin-2, ADAMTS-4 (aggrecanase-1), and stanniocalcin were revealed. Support for the biological significance was confirmed by the abrogation of the changes in the expression of angiogenesis inhibitors and in situ hybridization studies. This study has significantly extends the molecular fingerprint of the changes in gene expression that occur during endothelial differentiation and provides new insights into the potential role of a number of new molecules in angiogenesis. |
| Databáze: | OpenAIRE |
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