Distribution and Genetic Diversity of Grapevine Viruses in Russia
| Autor: | E. V. Porotikova, Uliana Terehova, Eugeniya Yurchenko, S.V. Vinogradova, Vitalii Volodin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Veterinary medicine viral diagnostics Plant Science 01 natural sciences Vineyard Article Virus grapevine virus infection 03 medical and health sciences grapevine viruses genetic variability Genetic variability Ecology Evolution Behavior and Systematics Phytosanitary certification Genetic diversity Vitis vinifera Ecology biology phylogenetic analysis molecular variants Botany Grapevine fanleaf virus biology.organism_classification 030104 developmental biology QK1-989 RFLP Viticulture Viral load 010606 plant biology & botany |
| Zdroj: | Plants Volume 10 Issue 6 Plants, Vol 10, Iss 1080, p 1080 (2021) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants10061080 |
| Popis: | Viral diseases can seriously damage the vineyard productivity and the quality of grape and wine products. Therefore, the study of the species composition and range of grapevine viruses is important for the development and implementation of strategies and tactics to limit their spread and increase the economic benefits of viticulture. In 2014–2019, we carried out a large-scale phytosanitary monitoring of Russian commercial vineyards in the Krasnodar region, Stavropol region and Republic of Crimea. A total of 1857 samples were collected and tested for the presence of Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine virus A (GVA), Grapevine leafroll-associated virus-1 (GLRaV-1), Grapevine leafroll-associated virus-2 (GLRaV-2), Grapevine leafroll-associated virus-3 (GLRaV-3), Grapevine fanleaf virus (GFLV), and Grapevine fleck virus (GFkV) using RT-PCR. Out of all samples tested, 54.5% were positive for at least one of the viruses (GRSPaV, GVA, GLRaV-1, GLRaV-2, GLRaV-3, GFLV, GFkV) in the Stavropol region, 49.8% in the Krasnodar region and 49.5% in the Republic of Crimea. Some plants were found to be infected with several viruses simultaneously. In the Republic of Crimea, for instance, a number of plants were infected with five viruses. In the Krasnodar region and the Republic of Crimea, 4.7% and 3.3% of the samples were predominantly infected with both GFkV and GRSPaV, whereas in the Stavropol region, 6% of the selected samples had both GLRaV-1 and GVA infections. We carried out a phylogenetic analysis of the coat protein genes of the detected viruses and identified the presence of GVA of groups I and IV, GRSPaV of groups BS and SG1, GLRaV-1 of group III, GLRaV-2 of groups PN and H4, GLRaV-3 of groups I and III. The results obtained make it possible to assess the viral load and the distribution of the main grapevine viruses on plantations in the viticultural zones of Russia, emphasizing the urgent need to develop and implement long-term strategies for the control of viral diseases of grapes. |
| Databáze: | OpenAIRE |
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