Human in vitro cell lines verification by minisatellite DNA restriction fragment length polymorphism
| Autor: | Danuta Duś, Elżbieta Wojdat, Piotr Kuśnierczyk, Magdalena Zielińska-Dawidziak, Anita Kość, Joanna Dubis, Mańczak M, Magdalena Prussak |
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| Rok vydání: | 1998 |
| Předmět: |
Cancer Research
Lymphoma B-Cell Biology Cell Line HaeIII chemistry.chemical_compound Tumor Cells Cultured medicine Humans Genetics Molecular biology Restriction enzyme Terminal restriction fragment length polymorphism Minisatellite Oncology chemistry Cell culture Colonic Neoplasms Mutation Microsatellite Restriction fragment length polymorphism HT29 Cells Polymorphism Restriction Fragment Length DNA Microsatellite Repeats medicine.drug |
| Zdroj: | Cancer Letters. 128:113-120 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/s0304-3835(98)00062-7 |
| Popis: | Four families of human in vitro cell lines were tested for minisatellite restriction fragment length polymorphism (RFLP) using multilocus probes MZ1.3 and/or 33.15 after digestion of DNA with restriction enzymes HinfI or HaeIII. These results confirmed that (i) the RFLP pattern is relatively stable in established cell lines and, therefore, could be used as a specific marker of a cell line identity, (ii) the use of MZ1.3 and 33.15 probes permits the identification of hybridomas and (iii) one of the cell lines tested, a lymphoblastoid cell line HAJ, may possess a hot spot of mutation. |
| Databáze: | OpenAIRE |
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