Disc cell clusters in pathological human intervertebral discs are associated with increased stress protein immunostaining
Autor: | Sally Roberts, Helena Evans, Sharon J. Brown, CA Sharp |
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Rok vydání: | 2009 |
Předmět: |
Adult
Pathology medicine.medical_specialty Adolescent HSP27 Heat-Shock Proteins HSP72 Heat-Shock Proteins Degenerative disc disease Young Adult Heat Shock Transcription Factors Antigen Hsp27 Heat shock protein Extracellular Humans Medicine Orthopedics and Sports Medicine Intervertebral Disc Heat-Shock Proteins Aged Aged 80 and over biology business.industry Intervertebral disc Middle Aged medicine.disease Immunohistochemistry DNA-Binding Proteins medicine.anatomical_structure Case-Control Studies biology.protein Spinal Diseases Original Article Surgery business Immunostaining Molecular Chaperones Transcription Factors |
Zdroj: | European Spine Journal. 18:1587-1594 |
ISSN: | 1432-0932 0940-6719 |
DOI: | 10.1007/s00586-009-1053-2 |
Popis: | Intervertebral disc (IVD) cells within the annulus fibrosus (AF) and nucleus pulposus (NP) maintain distinct functional extracellular matrices and operate within a potentially noxious and stressful environment. How disc cells respond to stress and whether stress is responsible for triggering degeneration is unknown. Disc cell proliferation and cluster formation are most marked in degenerate IVDs, possibly indicating attempts at matrix repair. In other tissues, stress proteins increase rapidly after stress protecting cell function and, although implicated in degeneration of articular cartilage, have received little attention in degenerative IVD pathologies. We have compared the distribution of stress protein immunolocalization in pathological and control IVDs. Disc tissues were obtained at surgery from 43 patients with degenerative disc disease (DDD) and herniation, and 12 controls at postmortem. Tissues were immunostained with a polyclonal antibody for heat shock factor 1 (HSF-1) and monoclonal antibodies for the heat shock proteins, Hsp27 and Hsp72, using an indirect immunoperoxidase method. Positively stained cells were expressed as a percentage of the total. Cell cluster formation was also assessed. The proportion of cells in clusters was similar in the AF (both 2%) and NP (8 and 9%) of control and DDD samples, whereas in herniated tissues this was increased (AF 12%, NP 14%). Stress antigen staining tended to be more frequent in clustered rather than in single/doublet cells, and this was significant (P |
Databáze: | OpenAIRE |
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