Differential expression of the Kell blood group and CD10 antigens: two related membrane metallopeptidases during differentiation of K562 cells by phorbol ester and hemin
| Autor: | Yves Colin, Sandrine Guérin, Patrick Auberger, Bernard Mari, Nathalie Belhacene, Jean-Ehrland Ricci, Laurence Maulon, Jean-Pierre Cartron |
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| Rok vydání: | 1998 |
| Předmět: |
Transcription
Genetic Biology Biochemistry chemistry.chemical_compound Antigen Antigens CD hemic and lymphatic diseases Tumor Cells Cultured Genetics Humans RNA Messenger Molecular Biology Protein kinase C Messenger RNA Kell Blood-Group System Cell growth Cell Membrane Cell Differentiation Kell antigen system Molecular biology Gene Expression Regulation Neoplastic Kinetics chemistry Cell culture Hemin Tetradecanoylphorbol Acetate Neprilysin Leukemia Erythroblastic Acute Cell Division Biotechnology K562 cells |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fasebj.12.7.531 |
| Popis: | The erythroleukemic cell line K562 can undergo further differentiation in erythroid or megakaryocytic lineage depending on the nature of the stimulus. Phorbol ester (PMA) stimulates megakaryocytic development whereas hemin promotes erythroid differentiation of these cells. We have examined the effect of PMA and hemin on the expression of the Kell blood group and CD10 antigens, two related proteins that belong to a family of membrane-bound neutral metalloendopeptidases. We show here that differentiation of K562 cells by PMA in the megakaryocytic lineage results in abolishment of Kell mRNA accumulation and protein expression and, in parallel, the induction of CD10 mRNA accumulation, protein expression, and enzymatic activity. Conversely, differentiation of these cells by hemin in the erythroid lineage is accompanied by an up-regulation of Kell mRNA and protein expression, with no changes in CD10 mRNA and protein expression. Thus, CD10 and Kell can be regarded as specific markers of the differentiation of K562 cells in the megakaryocytic and erythroid lineages, respectively. |
| Databáze: | OpenAIRE |
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