A 6-month inhalation toxicology study in Apoe−/− mice demonstrates substantially lower effects of e-vapor aerosol compared with cigarette smoke in the respiratory tract
Autor: | Walter K. Schlage, Julia Hoeng, Dariusz Peric, Nicolas Sierro, John H. Miller, Ee Tsin Wong, Blaine Phillips, Justyna Szostak, Jingjie Zhang, Emmanuel Guedj, Patrick Vanscheeuwijck, Jovan Simicevic, Tom Lee, Manuel C. Peitsch, Patrice Leroy, Remi Dulize, Maica Corciulo, Oksana Lavrynenko, Yang Xiang, Kyeonghee Monica Lee, David Bornand, Bjoern Titz, Sin Kei Wong, Karsta Luettich, Mohamed Amin Choukrallah, Arkadiusz K. Kuczaj, Guo Jie Loh, Thomas Schneider, Nikolai V. Ivanov, Mehdi Auberson, Celine Merg, Ansgar Buettner |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis 010501 environmental sciences Electronic Nicotine Delivery Systems Toxicology medicine.disease_cause 01 natural sciences law.invention Nicotine Mice law Smoke Respiratory system Electronic cigarette Lung COPD Inhalation Exposure Smoking General Medicine Tobacco Products respiratory system Respiratory Function Tests medicine.anatomical_structure Female Irritation medicine.drug medicine.medical_specialty complex mixtures Organ Toxicity and Mechanisms 03 medical and health sciences Apolipoproteins E Internal medicine Tobacco medicine Animals 0105 earth and related environmental sciences Emphysema Inflammation Aerosols business.industry medicine.disease 030104 developmental biology Endocrinology business Transcriptome Oxidative stress Respiratory tract |
Zdroj: | Archives of Toxicology |
ISSN: | 1432-0738 0340-5761 |
Popis: | Cigarette smoking is the major cause of chronic obstructive pulmonary disease. Considerable attention has been paid to the reduced harm potential of nicotine-containing inhalable products such as electronic cigarettes (e-cigarettes). We investigated the effects of mainstream cigarette smoke (CS) and e-vapor aerosols (containing nicotine and flavor) generated by a capillary aerosol generator on emphysematous changes, lung function, and molecular alterations in the respiratory system of female Apoe−/− mice. Mice were exposed daily (3 h/day, 5 days/week) for 6 months to aerosols from three different e-vapor formulations—(1) carrier (propylene glycol and vegetable glycerol), (2) base (carrier and nicotine), or (3) test (base and flavor)—or to CS from 3R4F reference cigarettes. The CS and base/test aerosol concentrations were matched at 35 µg nicotine/L. CS exposure, but not e-vapor exposure, led to impairment of lung function (pressure–volume loop area, A and K parameters, quasi-static elastance and compliance) and caused marked lung inflammation and emphysematous changes, which were confirmed histopathologically and morphometrically. CS exposure caused lung transcriptome (activation of oxidative stress and inflammatory responses), lipidome, and proteome dysregulation and changes in DNA methylation; in contrast, these effects were substantially reduced in response to the e-vapor aerosol exposure. Compared with sham, aerosol exposure (carrier, base, and test) caused a slight impact on lung inflammation and epithelia irritation. Our results demonstrated that, in comparison with CS, e-vapor aerosols induced substantially lower biological and pathological changes in the respiratory tract associated with chronic inflammation and emphysema. Supplementary Information The online version supplementary material available at 10.1007/s00204-021-03020-4. |
Databáze: | OpenAIRE |
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