A 6-month inhalation toxicology study in Apoe−/− mice demonstrates substantially lower effects of e-vapor aerosol compared with cigarette smoke in the respiratory tract

Autor: Walter K. Schlage, Julia Hoeng, Dariusz Peric, Nicolas Sierro, John H. Miller, Ee Tsin Wong, Blaine Phillips, Justyna Szostak, Jingjie Zhang, Emmanuel Guedj, Patrick Vanscheeuwijck, Jovan Simicevic, Tom Lee, Manuel C. Peitsch, Patrice Leroy, Remi Dulize, Maica Corciulo, Oksana Lavrynenko, Yang Xiang, Kyeonghee Monica Lee, David Bornand, Bjoern Titz, Sin Kei Wong, Karsta Luettich, Mohamed Amin Choukrallah, Arkadiusz K. Kuczaj, Guo Jie Loh, Thomas Schneider, Nikolai V. Ivanov, Mehdi Auberson, Celine Merg, Ansgar Buettner
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Health
Toxicology and Mutagenesis
010501 environmental sciences
Electronic Nicotine Delivery Systems
Toxicology
medicine.disease_cause
01 natural sciences
law.invention
Nicotine
Mice
law
Smoke
Respiratory system
Electronic cigarette
Lung
COPD
Inhalation Exposure
Smoking
General Medicine
Tobacco Products
respiratory system
Respiratory Function Tests
medicine.anatomical_structure
Female
Irritation
medicine.drug
medicine.medical_specialty
complex mixtures
Organ Toxicity and Mechanisms
03 medical and health sciences
Apolipoproteins E
Internal medicine
Tobacco
medicine
Animals
0105 earth and related environmental sciences
Emphysema
Inflammation
Aerosols
business.industry
medicine.disease
030104 developmental biology
Endocrinology
business
Transcriptome
Oxidative stress
Respiratory tract
Zdroj: Archives of Toxicology
ISSN: 1432-0738
0340-5761
Popis: Cigarette smoking is the major cause of chronic obstructive pulmonary disease. Considerable attention has been paid to the reduced harm potential of nicotine-containing inhalable products such as electronic cigarettes (e-cigarettes). We investigated the effects of mainstream cigarette smoke (CS) and e-vapor aerosols (containing nicotine and flavor) generated by a capillary aerosol generator on emphysematous changes, lung function, and molecular alterations in the respiratory system of female Apoe−/− mice. Mice were exposed daily (3 h/day, 5 days/week) for 6 months to aerosols from three different e-vapor formulations—(1) carrier (propylene glycol and vegetable glycerol), (2) base (carrier and nicotine), or (3) test (base and flavor)—or to CS from 3R4F reference cigarettes. The CS and base/test aerosol concentrations were matched at 35 µg nicotine/L. CS exposure, but not e-vapor exposure, led to impairment of lung function (pressure–volume loop area, A and K parameters, quasi-static elastance and compliance) and caused marked lung inflammation and emphysematous changes, which were confirmed histopathologically and morphometrically. CS exposure caused lung transcriptome (activation of oxidative stress and inflammatory responses), lipidome, and proteome dysregulation and changes in DNA methylation; in contrast, these effects were substantially reduced in response to the e-vapor aerosol exposure. Compared with sham, aerosol exposure (carrier, base, and test) caused a slight impact on lung inflammation and epithelia irritation. Our results demonstrated that, in comparison with CS, e-vapor aerosols induced substantially lower biological and pathological changes in the respiratory tract associated with chronic inflammation and emphysema. Supplementary Information The online version supplementary material available at 10.1007/s00204-021-03020-4.
Databáze: OpenAIRE
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