Antiproliferative Activity of 3-Aminobenzamide in A431 Carcinoma Cells Is Associated with a Target Effect on Cytoskeleton
Autor: | Elisabetta Straface, Maria Antonietta Croce, Walter Malorni, Miriam Capri, Daniela Monti, Gabriella Rainaldi, Claudio Franceschi, Roberta Tiozzo |
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Rok vydání: | 1996 |
Předmět: |
Cell Survival
Poly ADP ribose polymerase Cell Biophysics Poly(ADP-ribose) Polymerase Inhibitors Biochemistry chemistry.chemical_compound Tubulin Tumor Cells Cultured medicine Humans Cytotoxic T cell Enzyme Inhibitors Cytoskeleton Molecular Biology Polymerase chemistry.chemical_classification biology Cell growth fungi food and beverages Cell Biology Actins Cell biology medicine.anatomical_structure Enzyme chemistry 3-Aminobenzamide Benzamides biology.protein Cell Division |
Zdroj: | Biochemical and Biophysical Research Communications. 225:826-832 |
ISSN: | 0006-291X |
DOI: | 10.1006/bbrc.1996.1258 |
Popis: | 3-Aminobenzamide (3-ABA) is an inhibitor of poly(ADP-ribose)polymerase (PARP), an enzyme involved in several cellular processes, and exerts its effects by acting at the cytoskeleton level. Here we show that 3-ABA has an antiproliferative effect on the human carcinoma cell line A431, as measured by different assays. 3-ABA was capable of inhibiting cell growth as well as colony formation, this inhibitory effect is reversible. Morphological analyses showed a series of cellular alterations, such as a remarkable increase of dendritic-like protrusions, quite unusual in epithelial cells, and suggestive of a differentiative triggering. Immunocytochemical studies suggested that a major target of 3-ABA was indeed the cytoskeleton. These data, together with those of the literature, indicate that 3-ABA, depending on cell histotype and drug concentration, is a versatile drug capable of exerting antiproliferative and cytostatic effects as well as cytotoxic and antiapoptotic effects, processes sharing an important involvement of cytoskeleton. These unique characteristics of 3-ABA may be of interest for cancer research. |
Databáze: | OpenAIRE |
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