Prostaglandin E2 as a Regulator of Germ Cells during Ovarian Development
Autor: | Sharon L. Eddie, Richard A. Anderson, Henry N. Jabbour, Andrew J. Childs, Rosemary A. L. Bayne, Craig S. Collins |
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Rok vydání: | 2009 |
Předmět: |
Somatic cell
Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Biochemistry chemistry.chemical_compound Endocrinology Pregnancy Prostaglandin E2 Prostaglandin-E Synthases media_common Reverse Transcriptase Polymerase Chain Reaction Gene Expression Regulation Developmental General Medicine Immunohistochemistry Activins Intramolecular Oxidoreductases medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Female lipids (amino acids peptides and proteins) Prostaglandin E medicine.drug Adult medicine.medical_specialty media_common.quotation_subject Prostaglandin E2 receptor Prostaglandin Context (language use) Ovary Biology Dinoprostone Andrology Translational Highlights from Jcem Fetus Internal medicine medicine Humans Receptors Prostaglandin E Molecular Biology Ovulation Brain-Derived Neurotrophic Factor Biochemistry (medical) Receptors Prostaglandin E EP2 Subtype chemistry Oocytes Myeloid Cell Leukemia Sequence 1 Protein Receptors Prostaglandin E EP4 Subtype |
Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 94:4053-4060 |
ISSN: | 1945-7197 0021-972X |
Popis: | The formation of primordial follicles occurs during fetal life yet is critical to the determination of adult female fertility. Prior to this stage, germ cells proliferate, enter meiosis, and associate with somatic cells. Growth and survival factors implicated in these processes include activin A (INHBA), the neurotrophins BDNF and NT4 (NTF5), and MCL1. The prostaglandins have pleiotrophic roles in reproduction, notably in ovulation and implantation, but there are no data regarding roles for prostaglandins in human fetal ovarian development.The aim of the study was to investigate a possible role for prostaglandin (PG) E(2) in human fetal ovary development.In vitro analysis of ovarian development between 8 and 20 wk gestation was performed.The expression patterns of PG synthesis enzymes and the PGE(2) receptors EP2 and EP4 in the ovary were assessed, and downstream effects of PGE(2) on gene expression were analyzed.Ovarian germ cells express the PG synthetic enzymes COX2 and PTGES as well as the EP2 and EP4 receptors, whereas COX1 is expressed by ovarian somatic cells. Treatment in vitro with PGE(2) increased the expression of BDNF mRNA 1.7 +/- 0.16-fold (P = 0.004); INHBA mRNA, 2.1 +/- 0.51-fold (P = 0.04); and MCL1 mRNA, 1.15 +/- 0.06-fold (P = 0.04), but not that of OCT4, DAZL, VASA, NTF5, or SMAD3.These data indicate novel roles for PGE(2) in the regulation of germ cell development in the human ovary and show that these effects may be mediated by the regulation of factors including BDNF, activin A, and MCL1. |
Databáze: | OpenAIRE |
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