Prostaglandin E2 as a Regulator of Germ Cells during Ovarian Development

Autor: Sharon L. Eddie, Richard A. Anderson, Henry N. Jabbour, Andrew J. Childs, Rosemary A. L. Bayne, Craig S. Collins
Rok vydání: 2009
Předmět:
Somatic cell
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Clinical Biochemistry
Biochemistry
chemistry.chemical_compound
Endocrinology
Pregnancy
Prostaglandin E2
Prostaglandin-E Synthases
media_common
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Regulation
Developmental
General Medicine
Immunohistochemistry
Activins
Intramolecular Oxidoreductases
medicine.anatomical_structure
Proto-Oncogene Proteins c-bcl-2
Female
lipids (amino acids
peptides
and proteins)
Prostaglandin E
medicine.drug
Adult
medicine.medical_specialty
media_common.quotation_subject
Prostaglandin E2 receptor
Prostaglandin
Context (language use)
Ovary
Biology
Dinoprostone
Andrology
Translational Highlights from Jcem
Fetus
Internal medicine
medicine
Humans
Receptors
Prostaglandin E
Molecular Biology
Ovulation
Brain-Derived Neurotrophic Factor
Biochemistry (medical)
Receptors
Prostaglandin E
EP2 Subtype
chemistry
Oocytes
Myeloid Cell Leukemia Sequence 1 Protein
Receptors
Prostaglandin E
EP4 Subtype
Zdroj: The Journal of Clinical Endocrinology & Metabolism. 94:4053-4060
ISSN: 1945-7197
0021-972X
Popis: The formation of primordial follicles occurs during fetal life yet is critical to the determination of adult female fertility. Prior to this stage, germ cells proliferate, enter meiosis, and associate with somatic cells. Growth and survival factors implicated in these processes include activin A (INHBA), the neurotrophins BDNF and NT4 (NTF5), and MCL1. The prostaglandins have pleiotrophic roles in reproduction, notably in ovulation and implantation, but there are no data regarding roles for prostaglandins in human fetal ovarian development.The aim of the study was to investigate a possible role for prostaglandin (PG) E(2) in human fetal ovary development.In vitro analysis of ovarian development between 8 and 20 wk gestation was performed.The expression patterns of PG synthesis enzymes and the PGE(2) receptors EP2 and EP4 in the ovary were assessed, and downstream effects of PGE(2) on gene expression were analyzed.Ovarian germ cells express the PG synthetic enzymes COX2 and PTGES as well as the EP2 and EP4 receptors, whereas COX1 is expressed by ovarian somatic cells. Treatment in vitro with PGE(2) increased the expression of BDNF mRNA 1.7 +/- 0.16-fold (P = 0.004); INHBA mRNA, 2.1 +/- 0.51-fold (P = 0.04); and MCL1 mRNA, 1.15 +/- 0.06-fold (P = 0.04), but not that of OCT4, DAZL, VASA, NTF5, or SMAD3.These data indicate novel roles for PGE(2) in the regulation of germ cell development in the human ovary and show that these effects may be mediated by the regulation of factors including BDNF, activin A, and MCL1.
Databáze: OpenAIRE
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