A teleost bactericidal permeability-increasing protein-derived peptide that possesses a broad antibacterial spectrum and inhibits bacterial infection as well as human colon cancer cells growth
| Autor: | Hong-mei Liu, Chang-biao Wang, Shu-wen He, Guang-hua Wang, Chao Li, Qin-qin Gu, Min Zhang, Li-hui Liu, Dong-fang Hao, Ningqiu Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Fish Proteins
0301 basic medicine Immunology Antineoplastic Agents Microbial Sensitivity Tests Vibrio vulnificus Microbiology 03 medical and health sciences Animals Humans Amino Acid Sequence Vibrio alginolyticus biology Vibrio parahaemolyticus Bacterial Infections Blood Proteins 04 agricultural and veterinary sciences Bactericidal/permeability-increasing protein biology.organism_classification Peptide Fragments Anti-Bacterial Agents 030104 developmental biology Colonic Neoplasms Flatfishes 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Drug Screening Assays Antitumor Micrococcus luteus Bacterial outer membrane Antibacterial activity HT29 Cells Bacteria Antimicrobial Cationic Peptides Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 118:103995 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2021.103995 |
| Popis: | The bactericidal permeability-increasing protein (BPI) is a multifunctional cationic protein produced by neutrophils with antibacterial, antitumor, and LPS-neutralizing properties. In teleost, a number of BPIs have been reported, but their functions are very limited. In this study, an N-terminal peptide, BO18 (with 18 amino acids), derived from rock bream (Oplegnathus fasciatus) BPI, was synthesized and investigated for its antibacterial spectrum, action mechanism, immunoregulatory property as well as the inhibition effects on bacterial invasion and human colon cancer cells growth. The results showed that BO18 was active against Gram-positive bacteria Bscillus subiilis, Micrococcus luteus, and Staphylococcus aureus, as well as Gram-negative bacteria Vibrio alginolyticus, Vibrio litoralis, Vibrio parahaemolyticus and Vibrio vulnificus. BO18 treatment facilitated the bactericidal process of erythromycin and rifampicin by enhancing the permeability of the outer membrane. During its interaction with V. alginolyticus, BO18 exerted its antibacterial activity by destroying cell membrane integrity, penetrating into the cytoplasm and binding to genomic DNA and total RNA. In vitro analysis indicated BO18 could enhance the respiratory burst ability and regulate the expression of immune related genes of macrophages. In vivo detection showed the administration of fish with BO18 before bacterial infection significantly reduced pathogen dissemination and replication in tissues. In addition, BO18 exerted a cytotoxic effect on the growth of human colon cancer cells HT-29. Together, these results add new insights into the function of teleost BPIs, and support that BO18 is a novel and broad-spectrum antibacterial peptide with potential to apply in fighting pathogenic infection in aquaculture. |
| Databáze: | OpenAIRE |
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