Activation of alternative NF-κB signaling during recovery of disuse-induced loss of muscle oxidative phenotype
| Autor: | Nicholas A.M. Pansters, Annemie M. W. J. Schols, Harry R. Gosker, Ramon C. J. Langen, A.H.V. Remels |
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| Přispěvatelé: | Pulmonologie, RS: NUTRIM - R3 - Chronic inflammatory disease and wasting |
| Rok vydání: | 2014 |
| Předmět: |
Physiology
Endocrinology Diabetes and Metabolism Oxidative Phosphorylation Weight-Bearing Mice Random Allocation Gene expression GENE-EXPRESSION SOLEUS MUSCLE High Mobility Group Proteins NF-kappa B MITOCHONDRIAL BIOGENESIS Phenotype Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Muscular Disorders Atrophic DNA-Binding Proteins medicine.anatomical_structure DIFFERENTIATION Hindlimb Suspension Receptors Estrogen SKELETAL-MUSCLE Signal Transduction medicine.medical_specialty EXERCISE Oxidative phosphorylation Biology ERR-ALPHA ATROPHY Mitochondrial Proteins Immobilization Atrophy reloading Physiology (medical) Internal medicine medicine Animals skeletal muscle Muscle Skeletal Soleus muscle Myosin Heavy Chains Skeletal muscle medicine.disease Nf κb signaling Mice Inbred C57BL alternative nuclear factor-kappa B Disease Models Animal Endocrinology Mitochondrial biogenesis Gene Expression Regulation ADAPTATIONS TIBIALIS ANTERIOR peroxisome proliferator-activated receptor-gamma coactivator Biomarkers Transcription Factors |
| Zdroj: | American Journal of Physiology : Endocrinology and Metabolism, 306(6), E615-E626. American Physiological Society |
| ISSN: | 1522-1555 0193-1849 |
| Popis: | Physical inactivity-induced loss of skeletal muscle oxidative phenotype (OXPHEN), often observed in chronic disease, adversely affects physical functioning and quality of life. Potential therapeutic targets remain to be identified, since the molecular mechanisms involved in reloading-induced recovery of muscle OXPHEN remain incompletely understood. We hypothesized a role for alternative NF-κB, as a recently identified positive regulator of muscle OXPHEN, in reloading-induced alterations in muscle OXPHEN. Markers and regulators (including alternative NF-κB signaling) of muscle OXPHEN were investigated in gastrocnemius muscle of mice subjected to a hindlimb suspension/reloading (HLS/RL) protocol. Expression levels of oxidative phosphorylation subunits and slow myosin heavy chain isoforms I and IIA increased rapidly upon RL. After an initial decrease upon HLS, mRNA levels of peroxisome proliferator-activated receptor (PPAR)-γ coactivator (PGC) molecules PGC-1α and PGC-1β and mRNA levels of mitochondrial transcription factor A (Tfam) and estrogen-related receptor α increased upon RL. PPAR-δ, nuclear respiratory factor 1 (NRF-1), NRF-2α, and sirtuin 1 mRNA levels increased during RL although expression levels were unaltered upon HLS. In addition, both Tfam and NRF-1 protein levels increased significantly during the RL period. Moreover, upon RL, IKK-α mRNA and protein levels increased, and phosphorylation of P100 and subsequent processing to P52 were elevated, reflecting alternative NF-κB activation. We conclude that RL-induced recovery of muscle OXPHEN is associated with activation of alternative NF-κB signaling. |
| Databáze: | OpenAIRE |
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