BPI-ANCA is expressed in the airways of cystic fibrosis patients and correlates to platelet numbers and Pseudomonas aeruginosa colonization
| Autor: | Oonagh Shannon, Lisa I. Påhlman, Gisela Hovold, Ulrika Lindberg, Johanna K. Ljungberg |
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| Rok vydání: | 2020 |
| Předmět: |
Pulmonary and Respiratory Medicine
Adult DNA Bacterial Male Cystic Fibrosis Neutrophils Respiratory System Colony Count Microbial medicine.disease_cause Cystic fibrosis Flow cytometry Antibodies Antineutrophil Cytoplasmic 03 medical and health sciences Leukocyte Count 0302 clinical medicine Medicine Humans Platelet Colonization 030212 general & internal medicine Inflammation medicine.diagnostic_test business.industry Pseudomonas aeruginosa Platelet Count Autoantibody Sputum Blood Proteins medicine.disease humanities respiratory tract diseases Immunoglobulin A 030228 respiratory system Immunology Female medicine.symptom business Airway Antimicrobial Cationic Peptides |
| Zdroj: | Respiratory medicine. 170 |
| ISSN: | 1532-3064 |
| Popis: | Background Autoantibodies to bactericidal/permeability-increasing protein (BPI), BPI-ANCA, are often present in serum of patients with cystic fibrosis (CF), and correlate to airway colonization with Pseudomonas aeruginosa. The aim of the study was to investigate if BPI-ANCA IgA is also present in the airways of CF patients, and if its presence correlates with neutrophil counts, platelets, and P. aeruginosa DNA in sputum. Methods BPI-ANCA IgA was quantified in serum and sputum samples from adult CF patients (n = 45) by ELISA. Sputum neutrophil counts, platelets, and platelet-neutrophil complexes were assessed by flow cytometry, and P. aeruginosa DNA was analysed with RT-PCR. Results Serum BPI-ANCA IgA was present in 44% of the study participants, and this group also had significantly enhanced BPI-ANCA levels in sputum compared to serum negative patients. Sputum levels of BPI-ANCA IgA correlated with P. aeruginosa DNA (r = 0.63, p = 0.0003) and platelet counts in sputum (r = 0.60, p = 0.0002). Conclusions BPI-ANCA is expressed in the airways of CF patients and correlates with P. aeruginosa load and platelet counts, suggesting a link to airway inflammation and mucosal immunity. |
| Databáze: | OpenAIRE |
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