Leukocyte rolling in venules of striated muscle and skin is mediated by P-selectin, not by L-selectin
| Autor: | F. Roesken, K. Messmer, Dietmar Vestweber, A. Botzlar, Eberhard Uhl, R. Hecht, U. Jager, D. Nolte, P. Schmid |
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| Rok vydání: | 1994 |
| Předmět: |
P-selectin
Physiology Leukocyte Rolling Platelet Membrane Glycoproteins Cross Reactions Cell Line Mice Venules Physiology (medical) E-selectin Cell Adhesion Leukocytes Tumor Cells Cultured Animals L-Selectin Cell adhesion Skin Mice Hairless Mice Inbred BALB C biology Cell adhesion molecule Chemistry Microcirculation Muscles Antibodies Monoclonal Molecular biology Hairless P-Selectin Microscopy Fluorescence biology.protein L-selectin E-Selectin Cardiology and Cardiovascular Medicine Cell Adhesion Molecules Selectin |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1994.267.4.h1637 |
| Popis: | Leukocyte rolling in post-capillary venules is mediated by adhesion molecules of the selectin family expressed on both leukocytes (L-selectin) and endothelial cells (E- and P-selectin). With the use of intravital fluorescence microscopy, the effects of antibodies against these selectins were analyzed in the skinfold chamber model of BALB/c mice and the ear model of homozygous hairless mice (hr/hr) that permit chronic observation of striated muscle and skin microcirculation in awake animals, respectively. Mice were injected intravenously with monoclonal antibodies (MAb) to murine L-selectin and E-selectin and affinity-purified polyclonal antibodies to P-selectin. The antibodies, which are known to block cell adhesion, were tested by immunoprecipitation to selectively bind to L-, E-, or P-selectin. Leukocyte rolling was a constant finding in both microcirculation models in the absence of inflammatory stimuli. In both models, injection of anti-P-selectin antibodies completely prevented baseline leukocyte rolling over an observation period of 2 h (P < 0.01 vs. baseline), while no effects were seen after administration of either anti-L-selectin or anti-E-selectin MAb. Treatment with the isotype-matched control antibodies did not affect leukocyte rolling in either model. We conclude that leukocyte rolling in postcapillary venules of murine striated muscle and skin is a physiological process mediated via P-selectin, whereas L- and E-selectin appear not to play a significant role under these circumstances. |
| Databáze: | OpenAIRE |
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