Overexpression of a Eutrema salsugineum phosphate transporter gene EsPHT1;4 enhances tolerance to low phosphorus stress in soybean
| Autor: | Shaohui Yang, Yue Feng, Yue Zhao, Jingping Bai, Jiehua Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Bioengineering
Phosphorus General Medicine Plants Genetically Modified Applied Microbiology and Biotechnology Plant Roots Up-Regulation Plant Leaves Gene Expression Regulation Plant Stress Physiological Brassicaceae Phosphate Transport Proteins Soybeans Cloning Molecular Biotechnology Plant Proteins |
| Zdroj: | Biotechnology letters. 42(11) |
| ISSN: | 1573-6776 |
| Popis: | To enhance Pi absorption and utilization efficiency of soybean, a member of PHT1 gene family was isolated and characterized from E. salsugineum, which was a homologous gene of AtPHT1;4 and consequently designated as EsPHT1;4.Quantitative real-time PCR (qRT-PCR) analysis showed that the transcript level of EsPHT1;4 significantly increased both in roots and leaves of E. salsugineum under Pi deficient conditions. Furthermore, EsPHT1;4 was transferred to soybean cultivar "YD22" using an Agrobacterium-mediated cotyledonary-node transformation method. Overexpression of EsPHT1;4 in soybean not only promoted the increase of plant biomass and yield of transgenic plants upon low P stress, but also increased the accumulation and transportation of Pi from roots to leaves in the transgenic soybean lines.EsPHT1;4 was critical for controlling the accumulation and translocation of Pi in plants, and can be subsequently used as an effective foreign gene for the improvement of P use efficiency of crops by genetic manipulation. |
| Databáze: | OpenAIRE |
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