Functional analysis of Drosophila HSP70 promoter with different HSE numbers in human cells
| Autor: | Gali Pavlova, Ekaterina Yu. Rybalkina, I B Mertsalov, Nadezda Kust, E Savchenko, A. V. Revishchin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Molecular Sequence Data
lcsh:Medicine Transfection Mice Molecular Cell Biology Animals Humans HSP70 Heat-Shock Proteins Glial Cell Line-Derived Neurotrophic Factor Heat shock Promoter Regions Genetic Molecular Biology Techniques lcsh:Science Molecular Biology Gene Regulation of gene expression Multidisciplinary Base Sequence biology HEK 293 cells lcsh:R Temperature Brain Biology and Life Sciences Genetic Therapy Cell Biology Gene Therapy biology.organism_classification Molecular biology Hsp70 Transplantation HEK293 Cells Transgenic Engineering Drosophila lcsh:Q Drosophila melanogaster Genetic Engineering Research Article Biotechnology |
| Zdroj: | PLoS ONE, Vol 9, Iss 8, p e101994 (2014) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The activation of genetic constructs including the Drosophila hsp70 promoter with four and eight HSE sequences in the regulatory region has been described in human cells. The promoter was shown to be induced at lower temperatures compared to the human hsp70 promoter. The promoter activity increased after a 60-min heat shock already at 38 °C in human cells. The promoter activation was observed 24 h after heat shock for the constructs with eight HSEs, while those with four HSEs required 48 h. After transplantation of in vitro heat-shocked transfected cells, the promoter activity could be maintained for 3 days with a gradual decline. The promoter activation was confirmed in vivo without preliminary heat shock in mouse ischemic brain foci. Controlled expression of the Gdnf gene under a Drosophila hsp70 promoter was demonstrated. This promoter with four and eight HSE sequences in the regulatory region can be proposed as a regulated promoter in genetic therapeutic systems. |
| Databáze: | OpenAIRE |
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