Up-Regulation of Vasopressin mRNA in Paraventricular Hypophysiotrophic Neurons after Acute Immobilization Stress

Autor: Jozsef Z. Kiss, Viktor Bartanusz, Jean-Michel Aubry, Judit S. Baffi, Daniela Jezova
Rok vydání: 1993
Předmět:
Male
endocrine system
Vasopressin
medicine.medical_specialty
Corticotropin-Releasing Hormone
Vasopressins
Endocrinology
Diabetes and Metabolism
Neuropeptide
In situ hybridization
Biology
Sulfur Radioisotopes
RNA
Messenger/ biosynthesis
Immobilization
Cellular and Molecular Neuroscience
Endocrinology
Neurons/ metabolism
Downregulation and upregulation
Internal medicine
Gene expression
medicine
Animals
RNA
Messenger
Rats
Wistar
In Situ Hybridization
Neurons
Sulfur Radioisotopes/diagnostic use
Messenger RNA
Endocrine and Autonomic Systems
Vasopressins/ biosynthesis
Paraventricular Hypothalamic Nucleus/cytology/ metabolism
ddc:616.8
Rats
Up-Regulation
Corticotropin-Releasing Hormone/biosynthesis
medicine.anatomical_structure
Stress
Psychological/ metabolism
Hypothalamus
Autoradiography
Oligonucleotide Probes
Up-Regulation/ physiology
Nucleus
Stress
Psychological
hormones
hormone substitutes
and hormone antagonists
Paraventricular Hypothalamic Nucleus
Zdroj: Neuroendocrinology, Vol. 58, No 6 (1993) pp. 625-629
ISSN: 1423-0194
0028-3835
DOI: 10.1159/000126602
Popis: Cellular levels of vasopressin (VP) and corticotropin-releasing factor (CRF) mRNAs were determined in the rat hypothalamic paraventricular nucleus after acute immobilization stress. Messenger RNA levels were measured by in situ hybridization histochemistry using 35S-labeled synthetic DNA oligonucleotide probes and quantitative autoradiography. Analysis of histograms related to density values revealed that two hours after immobilization the average cellular VP and CRF mRNA levels were significantly increased (145 +/- 30.3 and 68 +/- 21.3%, respectively, above control values). Moreover, the number of VP-expressing parvicellular neurons was doubled in stressed rats relative to controls. These results indicate that single acute immobilization stress is sufficient to up-regulate VP as well as CRF mRNA production in the hypothalamic CRF-neurosecretory system.
Databáze: OpenAIRE
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