Toxicogenomic Profiling of 28 Nanomaterials in Mouse Airways

Autor: Richard D. Handy, Hannu Norppa, Pia Anneli Sofia Kinaret, Tiina Skoog, Marit Ilves, Henrik Wolff, Gerard Vales, Sergio Moya, Harri Alenius, Kai Savolainen, Piia Karisola, Juha Kere, Bengt Fadeel, Dario Greco, Joseph Ndika
Přispěvatelé: Tampere University, BioMediTech, Institute of Biotechnology, Helsinki Institute of Life Science HiLIFE, University of Helsinki, HUMI - Human Microbiome Research, Research Programs Unit, Juha Kere / Principal Investigator, Staff Services
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Glycosylation
General Chemical Engineering
Science
General Physics and Astronomy
Medicine (miscellaneous)
02 engineering and technology
010402 general chemistry
01 natural sciences
Biochemistry
Genetics and Molecular Biology (miscellaneous)
Toxicogenetics
Nanomaterials
Transcriptome
chemistry.chemical_compound
transcriptomics
Mice
In vivo
Animals
General Materials Science
Lung
Research Articles
nanomaterials
318 Medical biotechnology
immunotoxicity
Chemistry
General Engineering
021001 nanoscience & nanotechnology
airway exposure
3. Good health
0104 chemical sciences
Cell biology
Nanostructures
Mice
Inbred C57BL
Nanotoxicology
toxicogenomics
Toxicity
Models
Animal
PEGylation
nanoparticles
Female
3111 Biomedicine
nanotoxicology
221 Nano-technology
0210 nano-technology
Toxicogenomics
Research Article
Zdroj: Advanced Science
Advanced Science, Vol 8, Iss 10, Pp n/a-n/a (2021)
ISSN: 2198-3844
Popis: Toxicogenomics opens novel opportunities for hazard assessment by utilizing computational methods to map molecular events and biological processes. In this study, the transcriptomic and immunopathological changes associated with airway exposure to a total of 28 engineered nanomaterials (ENM) are investigated. The ENM are selected to have different core (Ag, Au, TiO2, CuO, nanodiamond, and multiwalled carbon nanotubes) and surface chemistries (COOH, NH2, or polyethylene glycosylation (PEG)). Additionally, ENM with variations in either size (Au) or shape (TiO2) are included. Mice are exposed to 10 µg of ENM by oropharyngeal aspiration for 4 consecutive days, followed by extensive histological/cytological analyses and transcriptomic characterization of lung tissue. The results demonstrate that transcriptomic alterations are correlated with the inflammatory cell infiltrate in the lungs. Surface modification has varying effects on the airways with amination rendering the strongest inflammatory response, while PEGylation suppresses toxicity. However, toxicological responses are also dependent on ENM core chemistry. In addition to ENM‐specific transcriptional changes, a subset of 50 shared differentially expressed genes is also highlighted that cluster these ENM according to their toxicity. This study provides the largest in vivo data set currently available and as such provides valuable information to be utilized in developing predictive models for ENM toxicity.
C57BL/6 mice are exposed by oropharyngeal aspiration to 28 different engineered nanomaterials (ENM). Histological and cytological evaluation together with transcriptomics analyses reveals the important effect of surface functionalization (amination, carboxylation, or pegylation) as well as the significant role of the ENM core chemistry.
Databáze: OpenAIRE
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