Spatio-temporal localization of selected pectic and arabinogalactan protein epitopes and the ultrastructural characteristics of explant cells that accompany the changes in the cell fate during somatic embryogenesis in Arabidopsis thaliana
| Autor: | Izabela Potocka, Izabela Dobrowolska, Ewa Kurczyńska, Kamila Godel |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Somatic Embryogenesis Techniques Somatic embryogenesis Physiology Cellular differentiation Arabidopsis Arabidopsis thaliana arabinogalactan proteins Plant Science Cell fate determination Biology Pluripotent 01 natural sciences Galactans Epitope 03 medical and health sciences Plant Cells Genetics Arabidopsis Proteins Totipotent Plant cell Cell biology 030104 developmental biology Ultrastructure Callus Pectins 010606 plant biology & botany Explant culture |
| Zdroj: | Plant physiology and biochemistry : PPB. 127 |
| ISSN: | 1873-2690 |
| Popis: | During somatic embryogenesis (SE), explant cells undergo changes in the direction of their differentiation, which lead to diverse cell phenotypes. Although the genetic bases of the SE have been extensively studied in Arabidopsis thaliana, little is known about the chemical characteristics of the wall of the explant cells, which undergo changes in the direction of differentiation. Thus, we examined the occurrence of selected pectic and AGP epitopes in explant cells that display different phenotypes during SE. Explants examinations have been supplemented with an analysis of the ultrastructure. The deposition of selected pectic and AGP epitopes in somatic embryos was determined. Compared to an explant at the initial stage, a/embryogenic/totipotent and meristematic/pluripotent cells were characterized by a decrease in the presence of AGP epitopes, b/the presence of AGP epitopes in differentiated cells was similar, and c/an increase of analyzed epitopes was detected in the callus cells. Totipotent cells could be distinguished from pluripotent cells by: 1/the presence of the LM2 epitope in the latest one, 2/the appearance of the JIM16 epitope in totipotent cells, and 3/the more abundant presence of the JIM7 epitope in the totipotent cells. The LM5 epitope characterized the wall of the cells that were localized within the mass of embryogenic domain. The JIM8, JIM13 and JIM16 AGP epitopes appeared to be the most specific for the callus cells. The results indicate a relationship between the developmental state of the explant cells and the chemical composition of the cell walls. |
| Databáze: | OpenAIRE |
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