Polydatin and polydatin-loaded chitosan nanoparticles attenuate diabetic cardiomyopathy in rats
| Autor: | Manal Abdul-Hamid, Sanaa R. Galaly, Adel Abdel-Moneim, Fatma Mostafa, Hanaa M. Mohamed |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Histology Antioxidant Diabetic Cardiomyopathies Physiology medicine.medical_treatment Real-Time Polymerase Chain Reaction medicine.disease_cause Antioxidants Diabetes Mellitus Experimental Superoxide dismutase Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Glucosides Internal medicine Diabetic cardiomyopathy Stilbenes medicine Animals Rats Wistar Glycated Hemoglobin Chitosan 030102 biochemistry & molecular biology biology Chemistry Cell Biology General Medicine Glutathione medicine.disease Immunohistochemistry Rats Metformin Oxidative Stress 030104 developmental biology Endocrinology biology.protein Nanoparticles Creatine kinase Oxidative stress medicine.drug |
| Zdroj: | Journal of Molecular Histology. 52:135-152 |
| ISSN: | 1567-2387 1567-2379 |
| Popis: | Hyperglycemia is associated with impairment of heart function. The current study aimed to investigate the ameliorative effect of polydatin-loaded chitosan nanoparticles (PD-CSNPs), polydatin (PD) and metformin (MET) on diabetic cardiomyopathy in rats. Rats divided into six groups; normal-control, diabetic-control, diabetic + CSNPs (diabetic rats treated with 50 mg/kg blank chitosan nanoparticles), diabetic + PD-CSNPs (diabetic rats treated with PD-CSNPs equivalent to 50 mg/kg of polydatin), diabetic + PD (diabetic rats given 50 mg/kg polydatin), diabetic + MET (diabetic rats given 100 mg/kg metformin), orally and daily for 4 weeks. Treatment of diabetic rats with PD-CSNPs, PD and MET showed a significant reduction in the values of glucose and glycosylated hemoglobin with improvement in heart function biomarkers through decreasing serum creatine kinase and creatine kinase myocardial band activities compared to diabetic control. The treatment agents also suppressed the elevated lipid peroxidation product, increased values of glutathione content, superoxide dismutase, superoxide peroxidase, and catalase activities in the heart of diabetic treated rats. Furthermore, PD-CSNPs, PD and MET decreased heart tissue levels of a pro-inflammatory cytokine; tumor necrosis factor-alpha and nuclear factor-kappa β, upregulation of heart gene expressions; nuclear factor erythroid 2-related factor 2 and heme oxygenase-1. Histological and ultrastructural examinations revealed the ameliorative effect of PD-CSNPs, PD and MET against the harmful of diabetic cardiomyopathy by reducing the cardiac fibers, necrotic cardiac myocytes, inflammatory cell infiltration, and the arrangement of the myofibrils and intercalated discs. In conclusion, the new formula of PD-CSNPs was more effective than PD and MET in amelioration the diabetic cardiomyopathy through its antioxidant, anti-inflammatory and prolonged-release properties. |
| Databáze: | OpenAIRE |
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