Cloning and sequencing of protochlorophyllide reductase
| Autor: | W T Griffiths, P M Darrah, Steve A. Kay, Graham R. Teakle |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Oxidoreductases Acting on CH-CH Group Donors
Transcription Genetic Molecular Sequence Data Restriction Mapping Reductase Molecular cloning Biology Biochemistry Complementary DNA Genomic library Amino Acid Sequence Cloning Molecular Molecular Biology Peptide sequence Base Sequence cDNA library Hydrolysis Nucleic acid sequence Nucleic Acid Hybridization food and beverages DNA Cell Biology Blotting Northern Molecular biology Protochlorophyllide reductase Protein Biosynthesis RNA Edible Grain Oxidoreductases Research Article |
| Zdroj: | Biochemical Journal. 265:789-798 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2650789 |
| Popis: | Putative protochlorophyllide reductase cDNA clones (252 and 113) were isolated from an etiolated-oat (Avena sativa) cDNA library. These were used to indirectly characterize a further clone, p127, isolated from a lambda-phage gt11 cDNA library. The latter (1.15 kb in length) was sequenced, and the derived amino acid sequence was shown to be remarkably similar to that derived from chemical analysis of a CNBr-cleavage fragment of the purified reductase, p127 codes for more than 95% of the reductase protein. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|