Effect of IFN-gamma stimulation on expression of intercellular adhesion molecule-1 (ICAM-1) on alveolar macrophages in patients with non-small cell lung cancer
| Autor: | Marta Dabrowska, R Chazan, R Krenke, J Domagała-Kulawik, Grubek-Jaworska H, G Hoser |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Lung Neoplasms Immunology Intercellular Adhesion Molecule-1 Stimulation Flow cytometry Interferon-gamma Virology Carcinoma Non-Small-Cell Lung Macrophages Alveolar Carcinoma Medicine Humans Interferon gamma Lung cancer Cells Cultured Aged ICAM-1 medicine.diagnostic_test business.industry Cell Biology Middle Aged medicine.disease Recombinant Proteins respiratory tract diseases Gene Expression Regulation Neoplastic Bronchoalveolar lavage Case-Control Studies Female business Bronchoalveolar Lavage Fluid medicine.drug |
| Zdroj: | Journal of interferoncytokine research : the official journal of the International Society for Interferon and Cytokine Research. 26(3) |
| ISSN: | 1079-9907 |
| Popis: | An impairment of in vitro cytotoxicity and tumoricidal function of alveolar macrophages (AMs) in patients with lung cancer was reported in a number of studies. The aim of our study was to evaluate the expression of intercellular adhesion molecule-1 (ICAM-1) on AMs after stimulation with interferon-gamma (IFN-gamma) in patients with non-small cell lung cancer (NSCLC). The study was performed in 13 patients with NSCLC, 6 patients with various nonmalignant pulmonary diseases, and 6 healthy volunteers. AMs were isolated from bronchoalveolar lavage fluid (BALF) by adherence and then cultured with or without IFN-gamma for 24 h. The expression of ICAM-1 on AMs was analyzed by flow cytometry. Stimulation with IFN-gamma caused increased expression of ICAM-1 on AMs in all studied groups (p < 0.05). The degree of the increase in ICAM-1 expression on AMs after IFN-gamma stimulation was significantly lower in patients with NSCLC compared with healthy volunteers (p = 0.002) or the other patients (p = 0.022). The results suggest impaired reactivity of ICAM-1 expression on AMs after stimulation with IFN-gamma in patients with NSCLC, which might be involved in functional defects of AMs in patients with NSCLC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|