Ovariectomy induces oxidative stress and impairs bone antioxidant system in adult rats
| Autor: | Srinivasan Narasimhan, Arunakaran Jagadeesan, Balaganesh Muthusamy, Veni Subramaniam, Gopalakrishnan Vasudevan, Venkataraman Prabhu, Sridhar Muthusami, Ilangovan Ramachandran |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Antioxidant medicine.medical_treatment Ovariectomy Clinical Biochemistry medicine.disease_cause Biochemistry Bone resorption Antioxidants Bone and Bones Lipid peroxidation Superoxide dismutase chemistry.chemical_compound Internal medicine medicine Animals Bone Resorption Rats Wistar chemistry.chemical_classification Reactive oxygen species biology Superoxide Dismutase Glutathione peroxidase Biochemistry (medical) General Medicine Hydrogen Peroxide Rats Disease Models Animal Oxidative Stress Endocrinology chemistry biology.protein Ovariectomized rat Osteoporosis Female Lipid Peroxidation Oxidative stress |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 360(1-2) |
| ISSN: | 0009-8981 |
| Popis: | Background There is increasing evidence suggesting the role of free radicals in bone resorption and bone loss. Ovariectomized rats have been used as the animal model for the study of osteoporosis. Oxidative stress due to reactive oxygen species (ROS) can cause oxidative damage to cells. Cells have a number of defense mechanisms to protect themselves from the toxicity of ROS. Even though, there are studies portraying the role of free radicals in bone loss, the defense mechanism adapted by bone in ovariectomized animals remains obscure. We investigated the impact of ovariectomy (OVX) on the bone antioxidant system in rats. Methods Sixty days after bilateral OVX, the rats were killed and the femora were removed, the tissue homogenates were made and used for the estimation of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S transferase (GST), lipid peroxidation (LPO) and hydrogen peroxide (H2O2). Results The levels of LPO and H2O2 were increased and enzymatic antioxidants like SOD, GPx, GST were decreased in ovariectomized animals when compared to sham-operated control rats. The calculated correlation coefficient demonstrated strong correlation (0.905) between the production of H2O2 and LPO in the femur bone. A strong inverse (− 0.945) correlation was observed between H2O2 production and SOD activity. Conclusions OVX induces oxidative stress in the femur bone of rats. |
| Databáze: | OpenAIRE |
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