Growth inhibition of Listeria monocytogenes by a nonbacteriocinogenic Carnobacterium piscicola
| Autor: | Lilian Nilsson, Susanne Knøchel, Tina Beck Hansen, Anne Gravesen, Patricia Garrido, Carmen Buchrieser, Philippe Glaser, Lone Gram |
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| Rok vydání: | 2004 |
| Předmět: |
Purine
Bacteriological Techniques General Medicine Biology medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Listeria monocytogenes Propanediol Microbiology chemistry.chemical_compound Lactobacillus Regulon Glucose chemistry Biosynthesis Food Preservation Pyrimidine metabolism medicine Food Microbiology Growth inhibition Bacteria Biotechnology |
| Zdroj: | Journal of applied microbiology. 98(1) |
| ISSN: | 1364-5072 |
| Popis: | Aims: This study elucidates the mechanisms by which a nonbacteriocinogenic Carnobacterium piscicola inhibits growth of Listeria monocytogenes. Methods and Results: Listeria monocytogenes was exposed to live cultures of a bacteriocin-negative variant of C. piscicola A9b in co-culture, in a diffusion chamber system, and to a cell-free supernatant. Suppression of maximum cell density (0–3·5 log units) of L. monocytogenes was proportional to initial levels of C. pisciola (103–107 CFU ml−1). Cell-to-cell contact was not required to cause inhibition. The cell-free C. piscicola supernatant caused a decrease in L. monocytogenes maximum cell density, which was abolished by glucose addition but not by amino acid, vitamin or mineral addition. The fermentate also gave rise to a longer lag phase and a reduction in growth rate. These effects were independent of glucose and may have been caused by acetate production by C. piscicola. 2D gel-electrophoretic patterns of L. monocytogenes exposed to C. piscicola or to L. monocytogenes fermentate did not differ. Treatment with C. piscicola fermentate resulted in down-regulation (twofold) of genes involved in purine- or pyrimidine metabolism, and up-regulation (twofold) of genes from the regulon for vitamin B12 biosynthesis and propanediol and ethanolamine utilization. Conclusions: A nonbacteriocinogenic C. piscicola reduced growth of L. monocytogenes partly by glucose depletion. Significance and Impact of the Study: Understanding the mechanism of microbial interaction enhances prediction of growth in mixed communities as well as use of bioprotective principles for food preservation. |
| Databáze: | OpenAIRE |
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