Integrated Label-Free and 10-Plex DiLeu Isobaric Tag Quantitative Methods for Profiling Changes in the Mouse Hypothalamic Neuropeptidome and Proteome: Assessment of the Impact of the Gut Microbiome
| Autor: | Yatao Shi, Nicola Salvatore Orefice, Pingli Wei, Rui Liu, Dustin C. Frost, Yusi Cui, Caitlin Keller, Shuying Han, Junfeng Huang, Federico E. Rey, Lingjun Li, Tzu Wen L. Cross, Zihui Li |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Proteomics Proteome Hypothalamus Neuropeptide Endogeny Gut flora 010402 general chemistry 01 natural sciences Article Analytical Chemistry Mice Leucine Tandem Mass Spectrometry Animals Amines biology Chemistry Neuropeptides 010401 analytical chemistry Long-term potentiation biology.organism_classification Gastrointestinal Microbiome 0104 chemical sciences Cell biology Mice Inbred C57BL Isobaric labeling Models Animal Signal transduction |
| Zdroj: | Anal Chem |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Gut microbiota can regulate host physiological and pathological status through gut-brain communications or pathways. However, the impact of the gut microbiome on neuropeptides and proteins involved in regulating brain functions and behaviors is still not clearly understood. To address the problem, integrated label-free and 10-plex DiLeu isobaric tag-based quantitative methods were implemented to compare the profiling of neuropeptides and proteins in the hypothalamus of germ-free (GF)- vs conventionally raised (ConvR)-mice. A total of 2943 endogenous peptides from 63 neuropeptide precursors and 3971 proteins in the mouse hypothalamus were identified. Among these 368 significantly changed peptides (fold changes over 1.5 and a p-value of |
| Databáze: | OpenAIRE |
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