High-Throughput Screening of Chlorella Vulgaris Growth Kinetics inside a Droplet-Based Microfluidic Device under Irradiance and Nitrate Stress Conditions
Autor: | Noura S. Dosoky, Muhammad S. Khan, David R. Nobles, Marwa Gamal Saad, Hesham Shafik, Magdy I. El-Bana, Mohamed Shafick Zoromba, Laila Mekki |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Chlorella vulgaris
Microfluidics lcsh:QR1-502 chemistry.chemical_element biodiesel 02 engineering and technology 01 natural sciences Biochemistry droplet-based microfluidic device lcsh:Microbiology chemistry.chemical_compound Nitrate Bioenergy nitrate conventional batch cultures biomass productivity Molecular Biology Biodiesel Chromatography 010401 analytical chemistry 021001 nanoscience & nanotechnology Nitrogen 0104 chemical sciences chemistry Biofuel Biodiesel production 0210 nano-technology light |
Zdroj: | Biomolecules, Vol 9, Iss 7, p 276 (2019) Biomolecules Volume 9 Issue 7 |
Popis: | Biodiesel is an eco-friendly renewable fuel that can be derived from microalgae. Maximization of biomass and lipid productivities are considered the main challenges for algal biodiesel production. Since conventional batch cultures are time-, space-, and reagent-consuming with many restrictions to apply many replicates, microfluidic technology has recently emerged as an alternative low-cost and efficient technology with high throughput repeatability and reproducibility. Different applications of microfluidic devices in algal biotechnology have been reported, including cell identification, sorting, trapping, and metabolic screening. In this work, Chlorella vulgaris was investigated by encapsulating in a simple droplet-based micro-array device at different light intensities of 20, 80, and 200 µ mol/m2/s combined with different nitrate concentrations of 17.6, 8.8, and 4.4 mM. The growth results for C. vulgaris within microfluidic device were compared to the conventional batch culture method. In addition, the effect of combined stress of deficiencies in irradiance and nitrogen availability were studied to illustrate their impact on the metabolic profiling of microalgae. The results showed that the most optimum favorable culturing conditions for Chlorella vulgaris growth within the microfluidic channels were 17.6 mM and 80 µ mol/m2/s. |
Databáze: | OpenAIRE |
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