COX-2 and prostanoid expression in micturition pathways after cyclophosphamide-induced cystitis in the rat
| Autor: | Peter Zvara, Abbey Dattilio, Margaret A. Vizzard, Jeffrey B. Folsom, Vivian Y. Hu, Susan E. Malley |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Cyclophosphamide Physiology media_common.quotation_subject Urinary system Blotting Western Urinary Bladder Urination Inflammation urologic and male genital diseases Dinoprostone Drug Administration Schedule Immunoenzyme Techniques chemistry.chemical_compound Physiology (medical) Internal medicine Cystitis Prostaglandins Synthetic medicine Animals RNA Messenger Rats Wistar Furans media_common medicine.diagnostic_test Prostaglandin D2 Chemistry Cystometry Prostanoid Mast cell Immunohistochemistry Rats Isoenzymes Endocrinology medicine.anatomical_structure Gene Expression Regulation Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Female medicine.symptom medicine.drug |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 284:R574-R585 |
| ISSN: | 1522-1490 0363-6119 |
| DOI: | 10.1152/ajpregu.00465.2002 |
| Popis: | The purpose of this study was to determine the role of cyclooxygenase-2 (COX-2) and its metabolites in lower urinary tract function after induction of acute (4 h), intermediate (48 h), or chronic (10 day) cyclophosphamide (CYP)-induced cystitis. Bladders were harvested from euthanized female rats for analyses. Conscious cystometry was used to assess the effects of a COX-2-specific inhibitor, 5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl2(5 H)-furanone (DFU, 5 mg/kg sc), a disubstituted furanone, in CYP-induced cystitis. COX-2 mRNA was increased in inflamed bladders after acute (12-fold) and chronic (9-fold) treatment. COX-2 protein expression in inflamed bladders paralleled COX-2 mRNA expression. Prostaglandin D2-methoxime expression in the bladder was significantly ( P ≤ 0.01) increased in acute (3-fold) and chronic (5.5-fold) cystitis. Prostaglandin E2 was significantly ( P ≤ 0.01) increased (2-fold) in the bladder with intermediate (1.7-fold) and chronic (2.6-fold) cystitis. COX-2-immunoreactive cell profiles were distributed throughout the inflamed bladder and coexpressed histamine immunoreactivity. Conscious cystometry in rats treated with CYP + DFU showed increased micturition intervals 4 and 48 h after CYP treatment and decreased intravesical pressures during filling and micturition compared with rats treated with CYP + vehicle. These studies suggest an involvement of urinary bladder COX-2 and its metabolites in altered micturition reflexes with CYP-induced cystitis. |
| Databáze: | OpenAIRE |
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