Eumelanin Coated PLA Electrospun Micro Fibers as Bioinspired Cradle for SH-SY5Y Neuroblastoma Cells Growth and Maturation
| Autor: | Alessandro Pezzella, Federica Maria Caso, Luigi Ambrosio, Cosimo Carfagna, Maria Grazia Raucci, Irene Bonadies, Ines Fasolino, Francesca Cimino |
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| Přispěvatelé: | Fasolino, Ine, Bonadies, Irene, Ambrosio, Luigi, Raucci, Maria Grazia, Carfagna, Cosimo, Caso, Federica Maria, Cimino, Francesca, Pezzella, Alessandro |
| Rok vydání: | 2017 |
| Předmět: |
Materials science
neuronal cell growth Confocal Cellular differentiation neurodegenerative applications tissue engineered scaffold Nanotechnology 02 engineering and technology 010402 general chemistry Cell Maturation Cell morphology 01 natural sciences eumelanin Neuroblastoma Cell Line Tumor Humans General Materials Science electrospinning Cell Proliferation Melanins Tissue Engineering Tissue Scaffolds biology Cell growth Cell Differentiation Adhesion 021001 nanoscience & nanotechnology 0104 chemical sciences Cell biology Tubulin Cell culture neurodegenerative application biology.protein Materials Science (all) 0210 nano-technology |
| Zdroj: | ACS applied materials & interfaces 9 (2017): 40070–40076. doi:10.1021/acsami.7b13257 info:cnr-pdr/source/autori:Fasolino, Ines; Bonadies, Irene; Ambrosio, Luigi; Raucci, Maria Grazia; Carfagna, Cosimo; Caso, Federica Maria; Cimino, Francesca; Pezzella, Alessandro/titolo:Eumelanin Coated PLA Electrospun Micro Fibers as Bioinspired Cradle for SH-SY5Y Neuroblastoma Cells Growth and Maturation/doi:10.1021%2Facsami.7b13257/rivista:ACS applied materials & interfaces (Print)/anno:2017/pagina_da:40070/pagina_a:40076/intervallo_pagine:40070–40076/volume:9 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.7b13257 |
| Popis: | Within the framework of neurodegenerative disorder therapies, the fabrication of 3D eumelanin architectures represents a novel strategy to realize tissue-engineering scaffolds for neuronal cell growth and control by providing both mechanical support and biological signals. Here, an appropriate procedure combining electrospinning, spin coating and solid-state polymerization process is established to realize the scaffolds. For biological analysis, a human derived cell line SH-SY5Y from neuroblastoma is used. Cell maturation on eumelanin microfibers, random and aligned, is evaluated by using confocal analysis and specific markers of differentiating neurons (beta III tubulin and GAP-43 expression). Cell morphology is tested by SEM analysis and immunofluorescence techniques. As results, eumelanin coated microfibers prove capable to support biological response in terms of cell survival, adhesion and spreading and to promote cell differentiation toward a more mature neuronal phenotype as confirmed by GAP-43 expression over the culture. |
| Databáze: | OpenAIRE |
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