Identification of cellular proteins interacting with equine infectious anemia virus S2 protein
| Autor: | Susan Payne, Bich-Ty Gno, Lina Covaleda, Fredrick J. Fuller |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cancer Research
Virulence Factors viruses Protein subunit Molecular Sequence Data Article Virus Equine infectious anemia Viral Proteins Two-Hybrid System Techniques Virology Protein Interaction Mapping Animals Humans Immunoprecipitation biology Alternative splicing Signal transducing adaptor protein Equidae Sequence Analysis DNA biology.organism_classification Open reading frame Infectious Diseases Viral replication Host-Pathogen Interactions Lentivirus Infectious Anemia Virus Equine |
| Zdroj: | Virus Research. 151:235-239 |
| ISSN: | 0168-1702 |
| Popis: | The macrophage-tropic lentivirus, equine infectious anemia virus (EIAV), encodes the small auxiliary protein S2 from a short open reading frame that overlaps the amino terminus of env EIAV S2 is dispensable for virus replication in cultured cells but is required for disease production. S2 is approximately 7 kDa and has no overall amino acid sequence homology to other cellular or viral proteins. Therefore it is likely that S2 plays a role as an adaptor protein. To further investigate S2 function we performed a yeast-2-hybrid screen to identify cellular proteins that interact with EIAV S2. The screen identified two human cellular proteins, amplified in osteosarcoma (OS-9) and proteasome 26S ATPase subunit 3 (PSMC3) that interact with S2. The equine homologues of these proteins were cloned and their interactions with S2 confirmed using co-immunoprecipitation assays. We identified two OS-9 isoforms that interact with S2 and a third splice variant that does not, indicating a region of OS-9 apparently required for the S2 interaction. The roles of these cellular proteins during EIAV infection have not been determined. |
| Databáze: | OpenAIRE |
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