Impairment of antiviral immune response and disruption of cellular functions by SARS-CoV-2 ORF7a and ORF7b

Autor: Tránsito García-García, Raúl Fernández-Rodríguez, Natalia Redondo, Ana de Lucas-Rius, Sara Zaldívar-López, Blanca Dies López-Ayllón, José M. Suárez-Cárdenas, Ángeles Jiménez-Marín, María Montoya, Juan J. Garrido
Přispěvatelé: [Garcia-Garcia, Transito] Univ Cordoba, Dept Genet, Immunogen & Mol Pathogenesis Grp, UIC Zoonoses & Emergent Dis ENZOEM, Cordoba, Spain, [Fernandez-Rodriguez, Raul] Univ Cordoba, Dept Genet, Immunogen & Mol Pathogenesis Grp, UIC Zoonoses & Emergent Dis ENZOEM, Cordoba, Spain, [Zaldivar-Lopez, Sara] Univ Cordoba, Dept Genet, Immunogen & Mol Pathogenesis Grp, UIC Zoonoses & Emergent Dis ENZOEM, Cordoba, Spain, [Suarez-Cardenas, Jose M.] Univ Cordoba, Dept Genet, Immunogen & Mol Pathogenesis Grp, UIC Zoonoses & Emergent Dis ENZOEM, Cordoba, Spain, [Jimenez-Marin, Angeles] Univ Cordoba, Dept Genet, Immunogen & Mol Pathogenesis Grp, UIC Zoonoses & Emergent Dis ENZOEM, Cordoba, Spain, [Garrido, Juan J.] Univ Cordoba, Dept Genet, Immunogen & Mol Pathogenesis Grp, UIC Zoonoses & Emergent Dis ENZOEM, Cordoba, Spain, [Garcia-Garcia, Transito] Maimonides Biomed Res Inst Cordoba IMIB, GA Res Grp 14, Cordoba, Spain, [Fernandez-Rodriguez, Raul] Maimonides Biomed Res Inst Cordoba IMIB, GA Res Grp 14, Cordoba, Spain, [Zaldivar-Lopez, Sara] Maimonides Biomed Res Inst Cordoba IMIB, GA Res Grp 14, Cordoba, Spain, [Suarez-Cardenas, Jose M.] Maimonides Biomed Res Inst Cordoba IMIB, GA Res Grp 14, Cordoba, Spain, [Jimenez-Marin, Angeles] Maimonides Biomed Res Inst Cordoba IMIB, GA Res Grp 14, Cordoba, Spain, [Garrido, Juan J.] Maimonides Biomed Res Inst Cordoba IMIB, GA Res Grp 14, Cordoba, Spain, [Redondo, Natalia] CSIC, Ctr Invest Biol Margarita Salas CIB, Mol Biomed Dept, Madrid 28040, Spain, [de Lucas-Rius, Ana] CSIC, Ctr Invest Biol Margarita Salas CIB, Mol Biomed Dept, Madrid 28040, Spain, [Lopez-Ayllon, Blanca Dies] CSIC, Ctr Invest Biol Margarita Salas CIB, Mol Biomed Dept, Madrid 28040, Spain, [Montoya, Maria] CSIC, Ctr Invest Biol Margarita Salas CIB, Mol Biomed Dept, Madrid 28040, Spain, Junta de Andaluca, European Commission, Spanish Ministry of Science project, Junta de Andalucía, Consejo Superior de Investigaciones Científicas (España), Ministerio de Ciencia e Innovación (España), García-García, Tránsito, Fernández-Rodríguez, Raúl, Redondo, Natalia, Lucas-Rius, Ana de, Zaldívar-López, Sara, Dies López-Ayllón, Blanca, Suárez-Cárdenas, José M., Jiménez-Marín, Ángeles, Montoya, María, Garrido, Juan J.
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Popis: 23 p.-8 fig.-1 tab.-1 graph. abst.
SARS-CoV-2, the causative agent of the present COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome, and some have been implicated in facilitating infection and pathogenesis through their interaction with cellular components. Among these proteins, accessory protein ORF7a and ORF7b functions are poorly understood. In this study, A549 cells were transduced to express ORF7a and ORF7b, respectively, to explore more in depth the role of each accessory protein in the pathological manifestation leading to COVID-19. Bioinformatic analysis and integration of transcriptome results identified defined canonical pathways and functional groupings revealing that after expression of ORF7a or ORF7b, the lung cells are potentially altered to create conditions more favorable for SARS-CoV-2, by inhibiting the IFN-I response, increasing proinflammatory cytokines release, and altering cell metabolic activity and adhesion. Based on these results, it is plausible to suggest that ORF7a or ORF7b could be used as biomarkers of progression in this pandemic.
This research work was funded by: Junta de Andalucía project CV20-20089 (Call for Research Projects on SARS-CoV-2 and COVID-19), the European Commission – NextGenerationEU (Regulation EU 2020/2094), through CSIC’s Global Health Platform (PTI Salud Global) and the Spanish Ministry of Science project PID2021-123390B-I00.
Databáze: OpenAIRE
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