Prime-boost Vaccination based on DNA and Protein-loaded Microspheres for Tuberculosis Prevention
| Autor: | Izaíra T. Brandão, Célio Lopes Silva, Maristela Ruberti, José Maciel Rodrigues Júnior, Sandra Aparecida dos Santos, Edson Garcia Soares, Karla De Melo Lima |
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| Rok vydání: | 2004 |
| Předmět: |
Time Factors
Chaperonins Polymers medicine.medical_treatment Immunization Secondary Pharmaceutical Science Priming (immunology) Microbiology Microsphere law.invention DNA vaccination Interferon-gamma Mice chemistry.chemical_compound Bacterial Proteins Polylactic Acid-Polyglycolic Acid Copolymer law Vaccines DNA medicine Animals Lactic Acid Particle Size Lung Tuberculosis Pulmonary Mice Inbred BALB C business.industry Chaperonin 60 Mycobacterium tuberculosis Antibodies Bacterial Microspheres Recombinant Proteins Trehalose dimycolate Vaccination Cytokine chemistry Recombinant DNA Cytokines business Polyglycolic Acid Spleen DNA |
| Zdroj: | Journal of Drug Targeting. 12:195-203 |
| ISSN: | 1029-2330 1061-186X |
| DOI: | 10.1080/10611860410001723126 |
| Popis: | We evaluated the use of a vaccine formulation based on a mixture of two different PLGA microspheres, composed by faster and slower release profiles, containing DNA encoding hsp65 and the recombinant hsp65 protein, respectively, aiming to DNA priming and protein boost after a single dose vaccination. The combination of PLGA50:50 microspheres containing DNA-hsp65 and trehalose dimycolate (TDM) with PLGA75:25 microspheres containing recombinant hsp65 (prime-boost Me) was able to induce high levels of anti-hsp65 specific antibodies. The serum levels of these specific antibodies remained high during 90 days after vaccination, whereas the DNA Me formulation based only in DNA-hsp65 plus TDM-loaded microspheres was not able to sustain the high antibody levels during the same period. Production of IFN-gamma was significant in animals vaccinated with both formulations, while the prime-boost Me vaccinated mice sustained higher levels of this cytokine during all the evaluation period. Thus, prime-boost strategy by using biodegradable microspheres seems to be a promising strategy to stimulate long-lasting immune response. |
| Databáze: | OpenAIRE |
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