Plasmodium falciparum: selective growth of subpopulations from field samples following in vitro culture, as detected by the polymerase chain reaction
| Autor: | Xin Ping Zhu, Suganya Viriyakosol, K. N. Brown, Aree Seugorn, G. Snounou, Napaporn Siripoon, W. Jarra |
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| Rok vydání: | 1994 |
| Předmět: |
Genotype
Immunology Population Molecular Sequence Data Plasmodium falciparum Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention law parasitic diseases Parasite hosting Animals Humans Malaria Falciparum education Polymerase chain reaction DNA Primers Electrophoresis Agar Gel education.field_of_study biology Base Sequence Nucleic acid sequence General Medicine DNA Protozoan biology.organism_classification Molecular biology Brood In vitro Infectious Diseases Protozoa Parasitology |
| Zdroj: | Experimental parasitology. 79(4) |
| ISSN: | 0014-4894 |
| Popis: | Analysis of the Plasmodium falciparum parasites circulating in the blood of infected persons frequently reveals the presence of two or more genetically distinct parasite populations. P. falciparum parasites cultured in vitro, from brood specimens collected in the field, are often used for biological, immunological, and drug-resistance investigations relating to the epidemiology in the area concerned or on the assumption that the parasites which grow in vitro are in general representative of all P. falciparum parasites. By using the polymerase chain reaction to detect and characterize a number of parasite polymorphic genes with great sensitivity, the composition of P. falciparum populations from 51 isolates were compared on the day of collection and following 2 months of in vitro culture. It was found that substantial changes in the parasite population profile could be detected in ca. 70% of the samples analyzed. The implications of this observation for studies using parasite isolates cultured in vitro are discussed. |
| Databáze: | OpenAIRE |
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