Interaction of casein kinase 1 delta (CK1δ) with post-Golgi structures, microtubules and the spindle apparatus
| Autor: | Uwe Knippschild, Martin Stöter, Lars Behrend, Gabriel Rutter, Marion Kurth, Jochen Heukeshoven, Wolfgang Deppert |
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| Rok vydání: | 2000 |
| Předmět: |
Cytoplasm
Histology Golgi Apparatus Mitosis Antineoplastic Agents tau Proteins Spindle Apparatus Polo-like kinase Biology Vinblastine Microtubules Cell Line Pathology and Forensic Medicine Mice chemistry.chemical_compound Tubulin Casein kinase 2 alpha 1 Animals Protein Isoforms Cells Cultured Cytoskeleton Etoposide Glutathione Transferase Nucleic Acid Synthesis Inhibitors Mice Inbred BALB C Nocodazole Cell Cycle 3T3 Cells Cell Biology General Medicine Phosphoproteins Antineoplastic Agents Phytogenic Clathrin Spindle apparatus Cell biology Biochemistry chemistry Gamma Rays Microtubule Proteins Stathmin Casein kinase 1 Casein kinase 2 Casein Kinases Microtubule-Associated Proteins Protein Kinases Cytokinesis DNA Damage |
| Zdroj: | European Journal of Cell Biology. 79:240-251 |
| ISSN: | 0171-9335 |
| DOI: | 10.1078/s0171-9335(04)70027-8 |
| Popis: | Members of the casein kinase 1 family of serine/threonine kinases are highly conserved from yeast to mammals and seem to play an important role in vesicular trafficking, DNA repair, cell cycle progression and cytokinesis. We here report that in interphase cells of various mammalian species casein kinase 1 delta (CK1delta) specifically interacts with the trans Golgi network and cytoplasmic, granular particles that associate with microtubules. Furthermore, at mitosis CK1delta is recruited to the spindle apparatus and the centrosomes in cells, which have been exposed to DNA-damaging agents like etoposide or gammairradiation. In addition, determination of the affinity of CK1delta to different tubulin isoforms in immunoprecipitation-Western analysis revealed a dramatically enhanced complex formation between CK1delta and tubulins from mitotic extracts after introducing DNA damage. The high affinity of CK1delta to the spindle apparatus in DNA-damaged cells and its ability to phosphorylate several microtubule-associated proteins points to a regulatory role of CK1delta at mitosis. |
| Databáze: | OpenAIRE |
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