Chagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results
| Autor: | Helio Moraes-Souza, Francisco Louzada-Neto, Márcia Maria Ferreira-Silva, Dante Mário Langhi-Júnior, Norival Késper-Júnior, José Orlando Bordin, Virmondes Rodrigues-Júnior, Marcelo Romanelli, Gilberto de Araújo Pereira, Wendell Sf Meira, Fernando Valadares Basques, Eufrosina Setsu Umezawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Chagas disease
medicine.medical_specialty Trypanosoma cruzi TESA-blot 030204 cardiovascular system & hematology Blood donors Gastroenterology Serology 03 medical and health sciences 0302 clinical medicine Internal medicine Immunology and Allergy Medicine Diseases of the blood and blood-forming organs Inconclusive results Immunodiagnosis biology business.industry Hematology Gold standard (test) medicine.disease biology.organism_classification Original Article RC633-647.5 business 030215 immunology |
| Zdroj: | Hematology, Transfusion and Cell Therapy Hematology, Transfusion and Cell Therapy, Vol 43, Iss 4, Pp 410-416 (2021) Hematology, Transfusion and Cell Therapy, Volume: 43, Issue: 4, Pages: 410-416, Published: 29 NOV 2021 |
| ISSN: | 2531-1387 2531-1379 |
| Popis: | Background The screening ofTrypanosoma cruzi-infected blood donors using two serological techniques frequently leads to conflicting results. This fact prompted us to evaluate the diagnostic performance of four “in-house” immunodiagnostic tests and two commercially available enzyme-linked immunosorbent assays (ELISAs). Material and Methods One hundred and seventy-nine blood donors, whose screening for Chagas disease was doubtful, underwent three in-house ELISAs, one in-house immunoblotting test (TESA-blot), and two commercial ELISAs (bioMerieux and Wiener) in an attempt to define the presence or absence of infection. Simultaneously, 29 donors with previous positive results from three conventional serological tests and 30 donors with constant negative results were evaluated. Results The ELISA-Wiener showed the highest rate in sensitivity (98.92%) and the ELISA-bioMerieux, the highest specificity (99.45%), followed by the TESA-blot, which showed superior performance, with lower false-negative (2.18%) and false-positive (1.12%) rates. In series, the combination composed of the TESA-blot and ELISA-bioMerieux showed slightly superior performance, with trifunctional protein deficiency (TFP) = 0.01%. Conclusion Our study confirms the high sensitivity and specificity of commercial kits. To confirm the presence or absence of T. cruzi infection, the combination of TESA-blot and ELISA-bioMerieux may be suggested as the best alternative. Individually, the TESA-blot performed the closest to the gold standard; however, it is not commercially available. |
| Databáze: | OpenAIRE |
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