Functional beta-cells derived from umbilical cord blood mesenchymal stem cells for curing rats with streptozotocin-induced diabetes mellitus
| Autor: | Hala Gabr, Anu V. Ranade, Mohamed Ahmed Eladl, Mohamed El-Sherbiny, Maha Guimei |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment 030204 cardiovascular system & hematology Mesenchymal Stem Cell Transplantation Umbilical cord Streptozocin Diabetes Mellitus Experimental Umbilical Cord Random Allocation 03 medical and health sciences 0302 clinical medicine In vivo Insulin-Secreting Cells Diabetes mellitus Internal medicine Animals Humans Insulin Medicine 030212 general & internal medicine business.industry Mesenchymal stem cell Mesenchymal Stem Cells General Medicine Fetal Blood medicine.disease Streptozotocin Rats Transplantation Endocrinology medicine.anatomical_structure Liver Original Article business Immunostaining medicine.drug |
| Zdroj: | Singapore Med J |
| ISSN: | 0037-5675 |
| Popis: | Introduction This study aimed to investigate the therapeutic response to injected human umbilical cord blood mesenchymal stem cells (UCBMSCs) among albino rats with streptozotocin (STZ)-induced diabetes mellitus. Methods Control group (GI; n = 25) rats were fed with standard rat diet. Rats with STZ-induced diabetes mellitus without (GII; n = 25) and with (GIII; n = 25) differentiated human UCBMSCs implantation were the test groups. Rats were sacrificed in Week 11 following implantation. Liver biopsies were sectioned and stained in order to highlight both the presence and function of impregnated cells in the liver tissue. Results Haematoxylin and eosin-stained sections in GI and GII rats showed normal liver architecture while GIII rats showed presence of cell clusters inside the liver tissue and around the central veins. Cell clusters with blue cytoplasm were present in sections in GIII rats but absent in GI and GII rats, indicating the presence of injected differentiated human UCBMSCs. The anti-human insulin immunostaining of GIII rats showed clusters of cells within the liver parenchyma and around central veins, indicating that these cells were active and secreting insulin. Conclusion UCBMSCs are proficient in differentiating into insulin-producing cells in vivo under specific conditions and, when transplanted into the liver of albino rats with STZ-induced diabetes mellitus, were able to secrete insulin and partially control the status of diabetes mellitus in rats. |
| Databáze: | OpenAIRE |
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