External Asymmetry and Pectoral Fin Loss in the Bamboo Sole (Heteromycteris japonica): Small-Sized Sole with Potential as a Pleuronectiformes Experimental Model
| Autor: | Hayato Yokoi, Masako Takagi, Susumu Uji, Makoto Mogi, Miki Kikuchi, Tohru Suzuki, Tadahisa Seikai, Yudai Saito, Shunya Nakamura, Qiran Chen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Bamboo animal structures Fin media_common.quotation_subject Zoology Biology 03 medical and health sciences Animals Metamorphosis Microinjection Phylogeny media_common Body Patterning Larva Hatching Pigmentation fungi Fish fin Metamorphosis Biological Anatomy 030104 developmental biology Flatfishes Animal Science and Zoology Developmental biology |
| Zdroj: | Zoological science. 34(5) |
| ISSN: | 0289-0003 |
| Popis: | Pleuronectiform fish develop marked external asymmetry in eye location and skin color at metamorphosis. The bamboo sole, Heteromycteris japonica, also exhibits loss of the pectoral fins at metamorphosis. Because of its small body size, short generation time, and long spawning season, we focused on the bamboo sole as an experimental model to investigate metamorphic asymmetry and pectoral fin loss during development. In the present study, we utilized a small-scale culture system to evaluate bamboo sole larvae and larval development, and a microinjection system for fertilized eggs. The culture system described here uses an 18 L culture tank for rotifers (the first diet for larvae) and 5 L plastic beakers for larval culture. Under this system, most larvae completed metamorphosis, including one-eye migration and pigmentation of the ocular side, by 23 days postfertilization (dpf) at 25°C. Larvae at density of 120–150 per liter were grown from hatching to 23 dpf with a survival ratio of 60–75% per beaker. Pectoral fins, including coracoid and disk cartilage, formed but were completely lost in late metamorphosis without formation of proximal radials and fin rays. The microinjection system designed here is adequate for the bamboo sole and allows injection of 100 one-cell-stage embryos per day. We expect that the culture and microinjection systems described here will facilitate the use of the bamboo sole as an experimental model organism in developmental biology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|