Rapid changes of light microscopic indices of osteoclast-bone relationships correlated with electron microscopy
| Autor: | Robert L. Schultz, Paul J. McMillan, Eugene E. Joseph, Leonard J. Deftos, Raymond A. Dewri |
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| Rok vydání: | 1989 |
| Předmět: |
Male
Cell type Time Factors Endocrinology Diabetes and Metabolism Statistics as Topic chemistry.chemical_element Osteoclasts Cell Communication Calcium Bone and Bones law.invention Endocrinology law Osteoclast Microscopy medicine Animals Orthopedics and Sports Medicine Close contact Chemistry Rats Inbred Strains Anatomy Rats Microscopy Electron medicine.anatomical_structure Biophysics Electron microscope |
| Zdroj: | Calcified tissue international. 44(6) |
| ISSN: | 0171-967X |
| Popis: | The relationships of tibial endosteal osteoclasts to bone surfaces were quantitatively evaluated during initiation of calcium repletion in calcium-deficient rats. To do this, indices of osteoclast-bone relationships obtained by light microscopy were devised and evaluated by comparing with those obtained by electron microscopy (EM). These indices are the percent of the osteoclast width that (1) exhibits markers indicative of a ruffled border, (2) is in close contact with bone, (3) is isolated from bone by other cell types, and (4) is separated from bone by intercellular material. The indices obtained by light microscopy were strongly correlated with similar indices obtained by EM and were equally sensitive but considerably easier to obtain. The ruffled border and contact index were significantly decreased by 3 hours after beginning the meal whereas cells of other types became interposed between the osteoclasts and the bone. |
| Databáze: | OpenAIRE |
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