Wnt/β-catenin signalling pathway following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide
| Autor: | Marcelo de Souza Silva, Celina Tijuko Fujiyama Oshima, Ana Carolina Cuzzuol Fracalossi, Daniel Araki Ribeiro |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Pathology medicine.medical_specialty Clinical Biochemistry Wnt1 Protein Biology medicine.disease_cause Wnt-5a Protein Pathology and Forensic Medicine Malignant transformation medicine Animals Rats Wistar Oral mucosa Fluorescent Antibody Technique Indirect Molecular Biology beta Catenin Tissue homeostasis Wnt signaling pathway 4-Nitroquinoline-1-oxide Epithelium Rats Tongue Neoplasms Wnt Proteins WNT5A Disease Models Animal medicine.anatomical_structure Carcinogens Carcinoma Squamous Cell Cancer research Signal transduction Carcinogenesis Precancerous Conditions Signal Transduction |
| Zdroj: | Experimental and Molecular Pathology. 88:176-183 |
| ISSN: | 0014-4800 |
| DOI: | 10.1016/j.yexmp.2009.11.004 |
| Popis: | The Wnt/beta-catenin signaling pathway plays an important role in development, tissue homeostasis, and regeneration. Inappropriate activation of the Wnt pathway is linked to a wide range of human cancers. The purpose of this study was to characterize the Wnt/beta-catenin signaling pathway as depicted by the expression of Wnt1, Frizzled-1, Wnt5a, Frizzled-5 and beta-catenin during 4NQO-induced rat tongue carcinogenesis by immunohistochemistry. Male Wistar rats were distributed into three groups of 10 animals each and treated with 4NQO solution at 50 ppm through their drinking water for 4, 12, and 20 weeks. Ten animals were used as control group. No histopathological abnormalities were induced in the epithelium after 4 weeks of carcinogen exposure; however, an overexpression of Wnt5a was noticed when compared to control group (p0.05). The Wnt1 showed significant differences (p0.05) in pre-neoplastic lesions at 12 weeks following carcinogen exposure. In well-differentiated squamous cell carcinoma induced after 20 weeks of treatment with 4NQO, Wnt1 was expressed in the majority of the dysplasic cells and tumor cells. This was statistically significant (p0.05). No significant differences (p0.05) were found in expression of Frizzled-1, Frizzled-5 or beta-catenin following oral carcinogenesis. Taken together, our results support the belief that expression of Wnt1 and Wnt5a is related to malignant transformation and conversion of oral mucosa. |
| Databáze: | OpenAIRE |
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