Persistence of SARS-CoV-2 RNA in the nasopharyngeal, blood, urine, and stool samples of patients with COVID-19: a hospital-based longitudinal study
Autor: | Mohammad Shenagari, Saman Maroufizadeh, Aydin Pourkazemi, Fariborz Mansour-Ghanaei, Issa Jahanzad, Mahmoud Khoshsorour, Lida Mahfoozi, Tofigh Yaghubi Kalurazi, Farahnaz Joukar, Mehrnaz Asgharnezhad, Alireza Jafarinezhad, Sonbol Taramian, Mohammadreza Naghipour, Ezat Hesni, Heydar Ali Balou |
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Rok vydání: | 2021 |
Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Longitudinal study RT-PCR Infectious and parasitic diseases RC109-216 Urine Iran Biology Virus Persistence (computer science) law.invention Persistence Feces 03 medical and health sciences 0302 clinical medicine Randomized controlled trial law Nasopharynx Virology Internal medicine medicine Humans Longitudinal Studies 030212 general & internal medicine Respiratory system Viral shedding Survival analysis Aged Aged 80 and over SARS-CoV-2 Research COVID-19 Middle Aged Survival Analysis Virus Shedding Hospitalization 030104 developmental biology Infectious Diseases COVID-19 Nucleic Acid Testing RNA Viral RNA Female |
Zdroj: | Virology Journal Virology Journal, Vol 18, Iss 1, Pp 1-9 (2021) |
ISSN: | 1743-422X |
Popis: | Background The persistence of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) RNA in the body fluids of patients with the novel coronavirus disease 2019 (COVID-19) may increase the potential risk of viral transmission. There is still uncertainty on whether the recommended quarantine duration is sufficient to reduce the risk of transmission. This study aimed to investigate the persistence of SARS-CoV-2 RNA in the nasopharyngeal, blood, urine, and stool samples of patients with COVID-19. Methods In this hospital-based longitudinal study, 100 confirmed cases of COVID-19 were recruited between March 2020 and August 2020 in Guilan Province, north of Iran. Nasopharyngeal, blood, urine, and stool samples were obtained from each participant at the time of hospital admission, upon discharge, 1 week after discharge, and every 2 weeks until all samples were negative for SARS-CoV-2 RNA by reverse transcription-polymerase chain reaction (RT-PCR) assay. A survival analysis was also performed to identify the duration of viral persistence. Results The median duration of viral RNA persistence in the nasopharyngeal samples was 8 days from the first positive RT-PCR result upon admission (95% CI 6.91–9.09); the maximum duration of viral shedding was 25 days from admission. Positive blood, urine, and stool RT-PCR results were detected in 24%, 7%, and 6% of the patients, respectively. The median duration of viral persistence in the blood, urine, and stool samples was 7 days (95% CI 6.07–7.93), 6 days (95% CI 4.16–8.41), and 13 days (95% CI 6.96–19.4), respectively. Also, the maximum duration of viral persistence in the blood, urine, and stool samples was 17, 11, and 42 days from admission, respectively. Conclusion According to the present results, immediately after the hospitalized patients were discharged, no evidence of viral genetic materials was found. Therefore, appropriate treatments were selected for the patients at this hospital. However, we recommend further investigations on a larger sample size in multi-center and prospective randomized controlled trials (RCTs) to evaluate the effects of different drugs on the shedding of the virus through body secretions. |
Databáze: | OpenAIRE |
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