Adipose-Derived Stem Cells Enhance Cancer Stem Cell Property and Tumor Formation Capacity in Lewis Lung Carcinoma Cells Through an Interleukin-6 Paracrine Circuit
Autor: | Hong-Jian Wei, Wei Hong Chen, Win Ping Deng, Bou Yue Peng, Jui Hua Lu, Hsin Hua Chou, Hen Yu Liu |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
STAT3 Transcription Factor Carcinogenesis medicine.medical_treatment Adipose tissue 03 medical and health sciences Paracrine signalling Carcinoma Lewis Lung 0302 clinical medicine Cancer stem cell Paracrine Communication medicine Animals STAT3 Cell Proliferation biology Cell growth Interleukin-6 Lewis lung carcinoma Mesenchymal Stem Cells Cell Biology Hematology Janus Kinase 2 Mice Inbred C57BL 030104 developmental biology Cytokine Adipose Tissue 030220 oncology & carcinogenesis Immunology Cancer research biology.protein Neoplastic Stem Cells Female Stem cell Stromal Cells Developmental Biology Signal Transduction |
Zdroj: | Stem cells and development. 25(23) |
ISSN: | 1557-8534 |
Popis: | Adipose-derived stem cells (ADSCs) are multipotent cells that have attracted much recent attention and emerged as therapeutic approaches in several medical fields. Although current knowledge of the biological impacts of ADSCs in cancer research is greatly improved, the underlying effects of ADSCs in tumor development remain controversial and cause the safety concerns in clinical utilization. Hence, we isolated primary ADSCs from the abdominal fat of mice and conducted interaction of ADSCs with Lewis lung carcinoma cells in culture and in mice to investigate the impacts of ADSCs on tumor development. Cytokine array and neutralizing antibody were further utilized to identify the key regulator and downstream signaling pathway. In this study, we demonstrated that ADSCs enhance the malignant characteristics of LLC1 cells, including cell growth ability and especially cancer stem cell property. ADSCs were then identified to promote tumor formation and growth in mice. We further determined that ADSC interaction with LLC1 cells stimulates increased secretion of interleukin-6 mainly from ADSCs, which then act in a paracrine manner on LLC1 cells to enhance their malignant characteristics. Interleukin-6 was also identified to regulate genes related to cell proliferation and cancer stem cell, as well as to activate JAK2/STAT3, a predominant interleukin-6-activated pathway, in LLC1 cells. Collectively, we demonstrated that ADSCs play a pro-malignant role in tumor development of Lewis lung carcinoma cells by particularly promoting cancer stem cell property through interleukin-6 paracrine circuit, which is important for safety considerations regarding the clinical application of ADSCs. |
Databáze: | OpenAIRE |
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