PP-22 promotes autophagy and apoptosis in the nasopharyngeal carcinoma cell line CNE-2 by inducing endoplasmic reticulum stress, downregulating STAT3 signaling, and modulating the MAPK pathway
| Autor: | Zhenhua Li, Guo‐Cai Wang, Yun‐Yun Tang, Jian-Wei Jiang, Gui‐Xiang Tan, Xin‐Ning Wang, Wan‐Jing Cen, Xi‐Cheng Wang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway STAT3 Transcription Factor Physiology p38 mitogen-activated protein kinases Clinical Biochemistry Apoptosis p38 Mitogen-Activated Protein Kinases 03 medical and health sciences 0302 clinical medicine Annexin Cell Line Tumor Autophagy Humans STAT3 Cell Proliferation Nasopharyngeal Carcinoma biology Kinase Chemistry Endoplasmic reticulum Nasopharyngeal Neoplasms Cell Biology Saponins Endoplasmic Reticulum Stress Cell biology 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Mitogen-Activated Protein Kinases Apoptosis Regulatory Proteins |
| Zdroj: | Journal of cellular physiology. 234(3) |
| ISSN: | 1097-4652 |
| Popis: | Paris polyphylla var. yunnanensis, named Chong Lou, is considered an antitumor substance. In this study, we investigated the effect of PP-22, a monomer purified from P. polyphylla var. yunnanensis, on the nasopharyngeal carcinoma cell line CNE-2 in vitro. The results showed that PP-22 could inhibit the proliferation of CNE-2 cells via the induction of apoptosis, with evidence of the characteristic morphological changes in the apoptosis in the nucleus and an increase in Annexin V-positive cells. In addition, we found that PP-22 could activate the p38 mitogen-activated protein kinase (MAPK) pathway and that this activation was reversed by SB203580, a specific inhibitor of the p38 MAPK pathway. In contrast, PP-22 promoted apoptosis via an intrinsic pathway, including the endoplasmic reticulum stress pathway, in a caspase-dependent manner. A further study showed that PP-22 also induced apoptosis by downregulating the signal transducers and activators of transcription 3 (STAT3) pathway, and the inhibitory effect was also confirmed by STAT3 small interfering RNA. In addition, PP-22 could promote autophagy by inhibiting the extracellular regulated protein kinases (ERK) pathway. And autophagy plays a protective role against apoptosis. Together, these data show that PP-22 promotes autophagy and apoptosis in the nasopharyngeal carcinoma CNE-2 cell line. |
| Databáze: | OpenAIRE |
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