Prevalence of Bovine Mastitis Pathogens in Bulk Tank Milk in China
| Autor: | Ya Jing Wang, Yanliang Bi, Yun Qin, Wei Sun, Shengli Li, Jaime Maldonado Garcia, Roger Guix Vallverdú, Zhijun Cao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Physiology Maternal Health Staphylococcus lcsh:Medicine Artificial Gene Amplification and Extension Mastitis Pathology and Laboratory Medicine medicine.disease_cause Polymerase Chain Reaction Animal Diseases 0403 veterinary science Medicine and Health Sciences Prevalence Streptococcus Agalactiae Staphylococcus Aureus lcsh:Science Mastitis Bovine Mammals Multidisciplinary Agriculture Ruminants 04 agricultural and veterinary sciences Corynebacterium bovis Bacterial Pathogens Body Fluids Dairying Milk Medical Microbiology Vertebrates Female Sample collection Pathogens Anatomy Research Article China Livestock 040301 veterinary sciences Biology Research and Analysis Methods Real-Time Polymerase Chain Reaction Arcanobacterium pyogenes Microbiology 03 medical and health sciences Bovines medicine Animals Bulk tank Molecular Biology Techniques Microbial Pathogens Molecular Biology Streptococcus uberis Bacteria lcsh:R Organisms Biology and Life Sciences Streptococcus Bovine Mastitis medicine.disease biology.organism_classification 030104 developmental biology Streptococcus agalactiae Amniotes Women's Health Cattle lcsh:Q Streptococcus dysgalactiae Zoology |
| Zdroj: | PLoS ONE, Vol 11, Iss 5, p e0155621 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The objectives of this study were to estimate the herd prevalence of major mastitis pathogens in bulk tank milk (BTM) in China dairy herds, to determine the relationship between the presence of mastitis pathogens and bulk tank milk somatic cell counts (BTSCC), and to investigate the impact of different dairy cattle farming modes and region on bacterial species. BTM samples collected from 894 dairy herds in China were examined for the presence of mastitis pathogens. The Flinders Technology Associates (FTA) cards were used for BTM sample collection, storage, and transportation and bacterial DNA amplification by real-time PCR. Among contagious pathogens, Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus dysgalactiae were detected in 50.1, 92.2, and 72.3% of the 894 BTM samples, respectively. Among environmental pathogens, E. coli, Streptococcus uberis, Enterococcus spp., Klebsiella spp., Serratia marcescens, Corynebacterium bovis, and Arcanobacterium pyogenes were detected in 28.6, 8.9, 35.7, 20.0, 1.3, 17.0, and 67.2% of the BTM samples, respectively. Staphylococcal β-lactamase gene was detected in 61.7% of the BTM samples. The presence of Staphylococcus aureus and Arcanobacterium pyogenes were significantly associated with high BTSCC, respectively. Significant differences were found in presence of Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus dysgalactiae in BTM sampled from the small household farms, dairy-farming communities, and large-scaled dairy farms. There were significant differences in the presence of Streptococcus agalactiae, Streptococcus dysgalactiae, Arcanobacterium pyogenes, staphylococcal β-lactamase gene, Staphylococcus spp., Klebsiella spp., Enterococcus spp., and Streptococcus uberis in BTM among Inner Mongolia, Heilongjiang, and Hebei province. In conclusion, contagious mammary pathogens are predominated among pathogens in BTM samples in China. |
| Databáze: | OpenAIRE |
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