Molecular cloning and characterization of LKv1, a novel voltage-gated potassium channel in leech
| Autor: | Yunxia Zhu, Chunfa Jie, Yueqiao Huang, Diana L. Walker, Shawn Ahmed, Kristen M. Johansen, Jamie L. Weiss, Joørgen Johansen, Jay Yang |
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| Rok vydání: | 1999 |
| Předmět: |
animal structures
Subfamily Patch-Clamp Techniques Potassium Channels Blotting Western Molecular Sequence Data Leech Membrane Potentials Cellular and Molecular Neuroscience Leeches Animals Patch clamp Shaker Amino Acid Sequence Cloning Molecular Phylogeny Membrane potential Neurons biology General Neuroscience Voltage-gated potassium channel biology.organism_classification Molecular biology Biological Evolution Immunohistochemistry Potassium channel Cell biology Electrophysiology Aplysia Shaker Superfamily of Potassium Channels Electrophoresis Polyacrylamide Gel Ion Channel Gating |
| Zdroj: | Journal of neurobiology. 38(2) |
| ISSN: | 0022-3034 |
| Popis: | We have cloned a novel voltage-gated K channel, LKv1, in two species of leech. The properties of LKv1 expressed in transiently transfected HEK293 cells is that of a delayed rectifier current. LKv1 may be a major modulator of excitability in leech neurons, since antibody localization studies show that LKv1 is expressed in the soma and axons of all neurons in both the central and peripheral nervous systems. Comparison of the biophysical and pharmacological properties of LKv1 with native voltage-gated conductances in leech neurons suggests that LKv1 may correspond to the previously characterized delayed rectifier current, IK. Phylogenetic analysis of LKv1 shows that it is related to the Shaker subfamily of voltage-gated K channels although it occupies a separate branch from that of the monophyletic Shaker clade composed of the flatworm, Aplysia, Drosophila, and mammalian Shaker homologs as well as from that of two recently identified Shaker-related K channels in jellyfish. Thus, this analysis indicates that this group of voltage-gated K channels contains several evolutionarily divergent lineages. © 1999 John Wiley & Sons, Inc. J Neurobiol 38: 287–299, 1999 |
| Databáze: | OpenAIRE |
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