Ginsenoside Rh2 Ameliorates Atopic Dermatitis in NC/Nga Mice by Suppressing NF-kappaB-Mediated Thymic Stromal Lymphopoietin Expression and T Helper Type 2 Differentiation
Autor: | Jun Hyoung Lee, Sun Chang Kim, Chang-Hao Cui, Sungjoo Park, Eunsu Ko, Jingang Hou, Myung Ho Kim |
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Rok vydání: | 2019 |
Předmět: |
CD4-Positive T-Lymphocytes
Male 0301 basic medicine Thymic stromal lymphopoietin Ginsenosides Down-Regulation ginsenoside ginseng Immunoglobulin E Article Catalysis Cell Line Dermatitis Atopic Proinflammatory cytokine Inorganic Chemistry Pathogenesis Mice 03 medical and health sciences Th2 Cells 0302 clinical medicine Immune system thymic stromal lymphopoietin Dinitrochlorobenzene Animals Humans Interleukin 8 Physical and Theoretical Chemistry Molecular Biology Spectroscopy Skin NF-κB pathway Th2 differentiation atopic dermatitis biology Chemistry Organic Chemistry NF-kappa B Cell Differentiation General Medicine In vitro Computer Science Applications Disease Models Animal 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Cancer research Cytokines Tumor necrosis factor alpha |
Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 24 |
ISSN: | 1422-0067 |
Popis: | Ginsenosides are known to have various highly pharmacological activities, such as anti-cancer and anti-inflammatory effects. However, the search for the most effective ginsenosides against the pathogenesis of atopic dermatitis (AD) and the study of the effects of ginsenosides on specific cytokines involved in AD remain unclear. In this study, ginsenoside Rh2 was shown to exert the most effective anti-inflammatory action on thymic stromal lymphopoietin (TSLP) and interleukin 8 in tumor necrosis factor-alpha and polyinosinic: polycytidylic acid induced normal human keratinocytes by inhibiting proinflammatory cytokines at both protein and transcriptional levels. Concomitantly, Rh2 also efficiently alleviated 2,4-dinitrochlorobenzene-induced AD-like skin symptoms when applied topically, including suppression of immune cell infiltration, cytokine expression, and serum immunoglobulin E levels in NC/Nga mice. In line with the in vitro results, Rh2 inhibited TSLP levels in AD mice via regulation of an underlying mechanism involving the nuclear factor &kappa B pathways. In addition, in regard to immune cells, we showed that Rh2 suppressed not only the expression of TSLP but the differentiation of naï ve CD4+ T-cells into T helper type 2 cells and their effector function in vitro. Collectively, our results indicated that Rh2 might be considered as a good therapeutic candidate for the alternative treatment of AD. |
Databáze: | OpenAIRE |
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