Direct Detection of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio alginolyticus from Clinical and Environmental Samples by a Multiplex Touchdown Polymerase Chain Reaction Assay
| Autor: | Ji-Fang Yin, Yan Wang, Ying-Jian Chen, Ai-Yan Liu, Ming-Qiang Lin, Hong-Qiu Yin, Cheng-Jin Hu, Ming-Yi Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Vibrio vulnificus Sensitivity and Specificity law.invention Microbiology 03 medical and health sciences law Vibrio species Environmental Microbiology Medicine Animals Humans Multiplex Polymerase chain reaction Vibrio alginolyticus Colony-forming unit Bacteriological Techniques biology business.industry Vibrio parahaemolyticus biology.organism_classification Infectious Diseases Vibrio Infections Surgery business Multiplex Polymerase Chain Reaction Bacteria |
| Zdroj: | Surgical infections. 19(1) |
| ISSN: | 1557-8674 |
| Popis: | Background: Vibrio vulnificus, V. alginolyticus, and V. parahaemolyticus are commonly and opportunistically pathogenic to humans. Methods: In this study, a novel multiple touchdown polymerase chain reaction method (MT-PCR) was developed to benefit rapid and simultaneous detection of the presence of the three Vibrio species from the enriched clinical and environmental samples. Results: The method showed a sensitivity of 104 colony forming units (CFU)/mL for V. vulnificus, 103 CFU/mL for V. parahaemolyticus and V. alginolyticus, and a specificity of 100% for all the three Vibrio species. All strains of the three Vibrio species were detected in the spiked samples artificially contaminated with reference strains and were identified directly from the enriched clinical and environmental samples within three hours by this MT-PCR assay. All the corresponding bacteria were isolated from these enriched samples in 48 hours by standard microbiologic procedures. Conclusions: This MT-PCR method, which can dete... |
| Databáze: | OpenAIRE |
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