Aqueous green tea infusion extracted by ultra-sonication method, but not by conventional method, facilitates GLUT4 membrane translocation in adipocytes which potently ameliorates high-fat diet-induced obesity
Autor: | Chang-Min Lee, Seung-Hee Nam, Jong-Bang Eun, Protiva Rani Das, Min-Jung Park, Young-Min Kim, Dong-Il Kim |
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Rok vydání: | 2020 |
Předmět: |
030309 nutrition & dietetics
Sonication Flavonoid Biophysics Chromosomal translocation Green tea extract Diet High-Fat 03 medical and health sciences chemistry.chemical_compound Mice 0404 agricultural biotechnology Adipocyte Adipocytes Animals Food science Obesity Pharmacology chemistry.chemical_classification 0303 health sciences biology Tea Chemistry Plant Extracts Extraction (chemistry) 04 agricultural and veterinary sciences Cell Biology 040401 food science Polyphenol biology.protein GLUT4 Food Science |
Zdroj: | Journal of food biochemistryREFERENCES. 45(1) |
ISSN: | 1745-4514 |
Popis: | Green tea contains bioactive compounds, such as polyphenols, responsible for its health-promoting effects, including antiobesity and antidiabetic effects. We previously reported that ultra-sonication extraction (UE) could efficiently increase the extraction yield of green tea compounds. In the present study, we found that the extract obtained using UE contained higher phenolic and flavonoid contents than that obtained using the conventional method. We therefore considered the extract as a bioactive metabolite-rich functional green tea extract (BMF-GTE), and tested its glucose-lowering effect by generating an adipocyte cell line stably expressing 7myc-GLUT4-GFP. We found that BMF-GTE treatment increased GLUT4 translocation to the plasma membrane. Moreover, BMF-GTE administration attenuated weight gain in mice fed a high-fat diet (HFD). Importantly, HFD-induced glucose tolerance was ameliorated in the mice receiving BMF-GTE. Therefore, we conclude that BMF-GTE worked against obesity and diabetes, at least partially, by enhancing GLUT4 translocation in adipocytes. PRACTICAL APPLICATIONS: As green tea is one of the most consumed beverages worldwide, its health effects have been widely tested. In our previous studies, we found that ultra-sonication extraction (UE) has the potential to increase the aqueous extraction yield of green tea compounds compared to conventional extraction techniques. In this study, we examined the biological effect of bioactive metabolite-rich functional green tea extract (BMF-GTE) obtained using UE; we observed that administering BMF-GTE lowered the body weight and increased insulin sensitivity in mice fed a high-fat diet, potentially by facilitating the membrane translocation of GLUT4 in adipocytes. Therefore, this study suggests that the extract obtained with UE had antiobesity and antidiabetic properties, indicative of a potential application of UE in maximizing the beneficial effects of green tea on human health. |
Databáze: | OpenAIRE |
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