B-cell populations are expanded in breast cancer patients compared with healthy controls
| Autor: | Naoki Niikura, Yuki Saito, Banri Tsuda, Yasuhiro Suzuki, Hirohito Miyako, Rin Ogiya, Yoshie Kametani, Kozue Yokoyama, Risa Oshitanai, Mayako Terao, Toru Morioka, Takuho Okamura, Yutaka Tokuda, Asuka Miyamoto |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Oncology Memory B cell Adult Male medicine.medical_specialty FACS B-Lymphocyte Subsets Breast Neoplasms Cell Separation CD38 CD19 03 medical and health sciences 0302 clinical medicine Breast cancer Internal medicine HER2 medicine Humans Pharmacology (medical) Radiology Nuclear Medicine and imaging B cell Aged Aged 80 and over biology business.industry CD24 Cancer Cell Differentiation General Medicine Middle Aged medicine.disease Flow Cytometry Antigens Differentiation B-Lymphocyte 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Original Article Female CD5 business Biomarkers |
| Zdroj: | Breast Cancer (Tokyo, Japan) |
| ISSN: | 1880-4233 1340-6868 |
| Popis: | Background Historically, humoral immunity was considered unimportant in anti-tumor immunity, and the differentiation and anti-tumor activity of B cells in breast cancer are poorly understood. However, it was recently discovered that B cells participate in tumor immunity through both antibody production and immunosuppressive mechanisms. We analyzed the expression of B-cell differentiation markers in detail using fluorescence-activated cell sorting to investigate the relationship between B-cell subsets and breast cancer etiology. Methods Blood samples were taken from breast cancer patients and healthy donors, and peripheral blood mononuclear cells were collected. B cells at various stages of differentiation were identified by the expression of combinations of the cell surface markers CD5, CD19, CD21, CD24, CD27, CD38, CD45, and IgD. Statistical analysis of the proportions of each B-cell subtype in the different patient groups was then performed. Results Twenty-seven breast cancer patients and 12 controls were considered. The proportion of total B cells was significantly higher in cancer patients than in controls (11.51 ± 2.059 vs 8.905 ± 0.379%, respectively; p = 0.001). Breast cancer patients were then classified as High-B or Low-B for further analysis. A significantly higher proportion of memory B cells was found in the High-B group than in the Low-B or control groups (p = 0.003 and p = 0.043, respectively). Conclusions Breast cancer patients generally have a higher proportion of B cells than healthy controls, but this is highly variable. Analysis of the major B-cell surface markers indicates that memory B cells in particular are significantly expanded, or more robust, in breast cancer patients. Electronic supplementary material The online version of this article (10.1007/s12282-017-0824-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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