Investigation of the AQP Family in Soybean and the Promoter Activity of TIP2;6 in Heat Stress and Hormone Responses

Autor: Zhi-Juan Feng, Na Liu, Gu-Wen Zhang, Sheng-Chun Xu, Ya-Ming Gong, Fu-Ge Niu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
GmTIP2
Arginine
01 natural sciences
heat stress
lcsh:Chemistry
chemistry.chemical_compound
Plant Growth Regulators
Gene Expression Regulation
Plant
transcript expression
Arabidopsis
Promoter Regions
Genetic
activated GUS
Abscisic acid
lcsh:QH301-705.5
Spectroscopy
Methyl jasmonate
Chromosome Mapping
food and beverages
General Medicine
Computer Science Applications
Cell biology
Phenotype
Multigene Family
Transgene
Aquaporin
Biology
Aquaporins
Catalysis
Article
Inorganic Chemistry
Evolution
Molecular
03 medical and health sciences
Physical and Theoretical Chemistry
soybean
Molecular Biology
Gene
promoter
Gene Expression Profiling
Organic Chemistry
biology.organism_classification
aquaporin
030104 developmental biology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Soybeans
Transcriptome
hormone cues
Salicylic acid
Heat-Shock Response
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 2, p 262 (2019)
International Journal of Molecular Sciences
ISSN: 1422-0067
Popis: Aquaporins (AQPs) are one diverse family of membrane channel proteins that play crucial regulatory roles in plant stress physiology. However, the heat stress responsiveness of AQP genes in soybean remains poorly understood. In this study, 75 non-redundant AQP encoding genes were identified in soybean. Multiple sequence alignments showed that all GmAQP proteins possessed the conserved regions, which contained 6 trans-membrane domains (TM1 to TM6). Different GmAQP members consisted of distinct Asn-Pro-Ala (NPA) motifs, aromatic/arginine (ar/R) selectivity filters and Froger’s positions (FPs). Phylogenetic analyses distinguished five sub-families within these GmAQPs: 24 GmPIPs, 24 GmTIPs, 17 GmNIPs, 8 GmSIPs, and 2 GmXIPs. Promoter cis-acting elements analyses revealed that distinct number and composition of heat stress and hormone responsive elements existed in different promoter regions of GmAQPs. QRT-PCR assays demonstrated that 12 candidate GmAQPs with relatively extensive expression in various tissues or high expression levels in root or leaf exhibited different expression changes under heat stress and hormone cues (abscisic acid (ABA), l-aminocyclopropane-l-carboxylic acid (ACC), salicylic acid (SA) and methyl jasmonate (MeJA)). Furthermore, the promoter activity of one previously functionally unknown AQP gene-GmTIP2;6 was investigated in transgenic Arabidopsis plants. The beta-glucuronidase (GUS) activity driven by the promoter of GmTIP2;6 was strongly induced in the heat- and ACC-treated transgenic plants and tended to be accumulated in the hypocotyls, vascular bundles, and leaf trichomes. These results will contribute to uncovering the potential functions and molecular mechanisms of soybean GmAQPs in mediating heat stress and hormone signal responses.
Databáze: OpenAIRE
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