Retinoblastoma: Expression of HLA-G
Autor: | Hema L. Ramkumar, Ayshwarya Subramanian, Subramanian Krishnakumar, Mohan Adithi, Nalini Venkatesan, Mallikarjuna Kandalam |
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Rok vydání: | 2006 |
Předmět: |
Male
Pathology medicine.medical_specialty Retinal Neoplasms Blotting Western Human leukocyte antigen Biology Immunoenzyme Techniques Immune system HLA Antigens HLA-G medicine Humans Immunology and Allergy Cytotoxic T cell Neoplasm Invasiveness Child HLA-G Antigens Retinoblastoma Histocompatibility Antigens Class I Antibodies Monoclonal Infant medicine.disease Histocompatibility Ophthalmology Tumor Escape Child Preschool Cancer research Immunohistochemistry Female |
Zdroj: | Ocular Immunology and Inflammation. 14:207-213 |
ISSN: | 1744-5078 0927-3948 |
Popis: | Human leukocyte antigen (HLA) mediates interactions of tumor cells with cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Retinoblastoma (RB) is the most common intraocular malignant tumor in childhood and is characterized by direct spread to the optic nerve and orbit as well as hematogenous and lymphatic spread. Earlier, we observed that invasive RB showed reduced HLA, which could contribute to its escape from the immune system. Little is known about the role of the nonclassical HLA molecule, HLA-G, in RB and its role in tumor escape mechanisms in RB.Forty archival paraffin-embedded RB tumors were analyzed for the expression of HLA-G by immunohistochemistry using a monoclonal antibody; fresh tumor samples were also subjected to Western blot analysis. There were 22 tumors with no invasion and 18 with invasion of the choroid/optic nerve. Immunoanalysis was performed based on the International Histocompatibility Working Group Project Description.HLA-G was negative in the non-neoplastic retina, reduced in 22/22 tumors with no invasion, and positive in 15/18 with invasion. The immunohistochemistry results were confirmed by Western blot analysis. The difference in expression between the two groups was significant ( p0.001). There was no correlation of HLA-G expression with differentiation of the tumors.Increased expression of HLA-G was observed in invasive RB. This preliminary observation deserves further investigation and may shed more light on the immune escape mechanisms of this tumor and thus enable novel therapeutic strategies. |
Databáze: | OpenAIRE |
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