Novel electrochemical dual-aptamer-based sandwich biosensor using molybdenum disulfide/carbon aerogel composites and Au nanoparticles for signal amplification
| Autor: | Lin-Xia Fang, Yang Liu, Ke-Jing Huang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Materials science
Aptamer Becaplermin Biomedical Engineering Biophysics Metal Nanoparticles Nanoparticle Nanotechnology Biosensing Techniques Nanocomposites chemistry.chemical_compound Limit of Detection Electrochemistry Humans Disulfides Composite material Molybdenum disulfide Molybdenum Detection limit Aerogel Electrochemical Techniques Proto-Oncogene Proteins c-sis General Medicine Aptamers Nucleotide Carbon chemistry Colloidal gold Gold Selectivity Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 71:171-178 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2015.04.031 |
| Popis: | A new electrochemical aptamer biosensor for the platelet-derived growth factor BB (PDGF-BB) detection has been developed based on the signal amplification of MoS 2 /carbon aerogel composites (MoS 2 /CA) and sandwich assay. A facile hydrothermal route assisted by l -cysteine was applied to synthesize CA incorporated flower-like MoS 2 with the large surface active sites and good conductivity. The electrochemical aptasensor was constructed by sandwiching the PDGF-BB between a glassy carbon electrode modified with thiol-terminated PDGF-BB aptamer-1 (Apt1)/gold nanoparticles (AuNPs)/MoS 2 /CA and the AuNPs with thiol-terminated PDGF-BB aptamer-2 (Apt2) and 6-ferrocenyl hexanethiol (Fc). Fc–AuNPs–Apt2 acted as tracer and AuNPs/MoS 2 /CA were utilized as the biosensor platform to immobilize a large amount of capture aptamers, owing to their layered structure and high surface-to-volume ratio. Based on the sandwich format, a dual signal amplification strategy had been successfully developed with a wide linear response in the range of 0.001–10 nM and a limit of detection of 0.3 pM. The developed assay demonstrated good selectivity and high sensitivity, indicating potential applications in bioanalysis and biomedicine. |
| Databáze: | OpenAIRE |
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