Reciprocal homer1a and homer2 isoform expression is a key mechanism for muscle soleus atrophy in spaceflown mice
| Autor: | Blottner, Dieter, Trautmann, Gabor, Furlan, Sandra, Gambara, Guido, Block, Katharina, Gutsmann, Martina, Sun, Lian-Wen, Worley, Paul F., Gorza, Luisa, Scano, Martina, Lorenzon, Paola, Vida, Imre, Volpe, Pompeo, Salanova, Michele |
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| Přispěvatelé: | Blottner, D., Trautmann, G., Furlan, S., Gambara, G., Block, K., Gutsmann, M., Sun, L. -W., Worley, P. F., Gorza, L., Scano, M., Lorenzon, P., Vida, I., Volpe, P., Salanova, M. |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Male
Hindlimb unloading QH301-705.5 Neuromuscular Junction Muscle Proteins Article Rats Sprague-Dawley Mice Homer Scaffolding Proteins Homer isoform switch Animals Protein Isoforms Rats Wistar Biology (General) Muscle Skeletal QD1-999 Weightlessness NMJ adaptation Space Flight Rats Mice Inbred C57BL Muscular Atrophy Chemistry Hindlimb Suspension Muscle atrophy Microgravity 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit |
| Zdroj: | International Journal of Molecular Sciences, Vol 23, Iss 75, p 75 (2022) International Journal of Molecular Sciences |
| Popis: | The molecular mechanisms of skeletal muscle atrophy under extended periods of either disuse or microgravity are not yet fully understood. The transition of Homer isoforms may play a key role during neuromuscular junction (NMJ) imbalance/plasticity in space. Here, we investigated the expression pattern of Homer short and long isoforms by gene array, qPCR, biochemistry, and laser confocal microscopy in skeletal muscles from male C57Bl/N6 mice (n = 5) housed for 30 days in space (Bion-flight = BF) compared to muscles from Bion biosatellite on the ground-housed animals (Bion ground = BG) and from standard cage housed animals (Flight control = FC). A comparison study was carried out with muscles of rats subjected to hindlimb unloading (HU). Gene array and qPCR results showed an increase in Homer1a transcripts, the short dominant negative isoform, in soleus (SOL) muscle after 30 days in microgravity, whereas it was only transiently increased after four days of HU. Conversely, Homer2 long-form was downregulated in SOL muscle in both models. Homer immunofluorescence intensity analysis at the NMJ of BF and HU animals showed comparable outcomes in SOL but not in the extensor digitorum longus (EDL) muscle. Reduced Homer crosslinking at the NMJ consequent to increased Homer1a and/or reduced Homer2 may contribute to muscle-type specific atrophy resulting from microgravity and HU disuse suggesting mutual mechanisms. |
| Databáze: | OpenAIRE |
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